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1. (Article ID: 7273)
Maity K, Mandal EK, Maity S, Gantait SK, Das D, Maiti S, Maiti TK, Sikdar SR, Islam SS
Chemical analysis and study of immunoenhancing and antioxidant property of a glucan isolated from an alkaline extract of a somatic hybrid mushroom of Pleurotus florida and Calocybe indica variety APK2
International Journal of Biological Macromolecules 49(4) (2011)
555-560
A water-soluble glucan isolated from an alkaline extract of fruit bodies of a somatic hybrid mushroom PCH9FB of Pleurotus florida and Calocybe indica var. APK2 strains showed antioxidant properties with immune activation of macrophage, splenocyte, and thymocyte. On the basis of acid hydrolysis, methylation, periodate oxidation and NMR studies ((1)H, (13)C, DEPT-135, DQF-COSY, TOCSY, NOESY, ROESY, HMQC, and HMBC) the structure of the repeating unit of the glucan was established as: structure.
glucan, NMR studies, Immunoactivation, antioxidant property, somatic hybrid PCH9FB mushroom
NCBI PubMed ID: 21703299Publication DOI: 10.1016/j.ijbiomac.2011.06.007Journal NLM ID: 7909578Publisher: Butterworth-Heinemann
Correspondence: Islam SS
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Institutions: Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore, West Bengal, India, Department of Biotechnology, Indian Institute of Technology (IIT) Kharagpur, Kharagpur, West Bengal, India, Division of Plant Biology, Bose Institute, C.I.T. Scheme VII M, Kolkata, West Bengal, India
Methods: 13C NMR, 1H NMR, periodate oxidation, NMR-2D, GC-MS, acid hydrolysis, GC, paper chromatography, NMR-1D, GPC, extraction, optical rotation measurement, determination of absolute configuration, determination of NO production, macrophage activity assay, antioxidant activity assay, splenocyte proliferation assay, thymocyte proliferation assay
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