CSDB: Search results

References to other databases: GTC:G66271HF, GlycomeDB:3793, CCSD:33408, CBank-STR:8759
Structural formula & atomic coordinates
Sweet-II 3D model
Show glycosyltransferases

The cell wall of Actinoplanes brasiliensis INA 3802 contains a polysaccharide which has an unusual structure. The peculiar structure of the polymer has been established by a nondestructive procedure which included 1H- and 13C NMR spectroscopy. The polysaccharide consists of six tetrasaccharide links having the following composition →6(α-D-Glcp-(1→4)-β-D-Glcp2,3NAcA-(1→4)-α-D-Glcp2,3NAc-(1→4)-β-D-Manp2,3NAcA-(1→. No structurally identical polymer of the procaryotic cell wall has so far been reported.

structure, polysaccharide, cell wall, teichuronic acid, Actinoplanes, diaminomannuronic acid, diaminoglucuronic acid, diaminoglucose

NCBI PubMed ID: 8155787
Journal NLM ID: 0376536
Publisher: Nauka/Interperiodica
Institutions: Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia, School of Biology, Lomonosov Moscow State University, Moscow, Russia, Max Planck Institute of Immunology, Freiburg, Germany, Institute of Novel Antibiotics, Russian Academy of Medical Sciences, Moscow, Russia
Methods: 13C NMR, 1H NMR, gel filtration, NMR-2D, sugar analysis, 31P NMR, paper chromatography, electrophoresis, acid degradation

Information on the prevalence, compositions, and structures of anionic carbohydrate-containing polymers of cell walls of Gram-positive bacteria is summarized. The data suggest that these polymers are important for normal functioning of bacterial cells and require further studies. Structural data on teichoic acids found in the literature published over the last few years are discussed. This is a very diverse class of polymers whose structure-specific pathways of degradation were studied and NMR spectra were examined. Unique comprehensive tables of 13C-NMR spectroscopic data (mainly obtained by the authors) on these polymers are given in the Appendix. Other tables summarize data on teichuronic acids, sugar-phosphate polymers, acid polysaccharides, and structural variants of bonds between acid polysaccharides and peptidoglycans known from the literature. Functions of anionic polymers and their possible chemotaxonomic applications are discussed

13C-NMR, NMR, structure, cell wall, composition, teichoic acid, teichuronic acid, Gram-positive, gram-positive bacteria, taxonomy

NCBI PubMed ID: 9360295
Journal NLM ID: 0376536
Publisher: Nauka/Interperiodica
Institutions: School of Biology, Lomonosov Moscow State University, Russia

References to other databases: GTC:G66271HF, CCSD:45265, CBank-STR:9965
Structural formula & atomic coordinates
Sweet-II 3D model
Show glycosyltransferases

The subject of the present review is the structural diversity and abundance of cell wall teichuronic and teichulosonic acids of representatives of the order Actinomycetales. Recently found teichulosonic acids are a new class of natural glycopolymers with ald-2-ulosonic acid residues: Kdn (3-deoxy-D-glycero-D-galacto-non-2-ulosonic acid) or di-N-acyl derivatives of Pse (5,7-diamino-3,5,7,9-tetradeoxy-L-glycero-L-manno-non-2-ulosonic or pseudaminic acid) as the obligatory component. The structures of teichuronic and teichulosonic acids are presented. Data are summarized on the occurrence of the glycopolymers of different nature in the cell wall of the studied actinomycetes. The biological role of the glycopolymers and their possible taxonomic implication are discussed. The comprehensive tables given in the Supplement show (13)C NMR spectroscopic data of teichuronic and teichulosonic acids obtained by the authors.

NMR spectroscopy, cell wall, teichuronic acid, actinomycetes, teichulosonic acid

NCBI PubMed ID: 21999534
Publication DOI: 10.1134/S0006297911070030
Journal NLM ID: 0376536
Publisher: Nauka/Interperiodica
Correspondence: em_tulskaya@mail.ru
Institutions: Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia, All-Russian Collection of Microorganisms (VKM), Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Russia, Lomonosov Moscow State University, Russia, Biological Faculty, Lomonosov Moscow State University, Moscow, Russia
Methods: NMR, ESI-MS, MALDI-TOF MS

The cell wall of Actinoplanes brasiliensis INA 3802 contains a teichuronic acid of unusual structure, as determined in a nondestructive way by 1H- and 13C-NMR spectroscopy. The polysaccharide comprises six tetrasaccharide units of the following composition: →6)-α-D-Glcp-(1→4)-β-D-2,3-diacetamido- 2,3-dideoxyGlcpA-(1→4)-α-D-2,3-diacetamido-2,3-dideoxyGlcp-(1→4)-β-D-2,3-diacetamido-2,3-dideoxyManpA. A polymer of such structure has not heretofore been reported for procaryotic cell walls.

NCBI PubMed ID: 7803461
Journal NLM ID: 0217513
Publisher: Elsevier
Institutions: N.D. Zelinsky Institute of Organic Chemistry, Academy of Sciences, Moscow, Russia