S-form lipopolysaccharide was isolated by phenol/water extraction from a strain of Acinetobacter calcoaceticus (DNA group 1). The structure of the O-antigenic polysaccharide was determined by compositional analysis and NMR spectroscopy of the de-O-acylated lipopolysaccharide. The isolated polysaccharide obtained after hydrolysis of lipopolysaccharide in 0.01 M trifluoroacetic acid has the followint structure: [see formula in text] in which Pyr is pyruvate. the O-acetyl substitution of D-Gal was non-stoichiometric. The O-antigen was specifically recognised in wesern blots by polyclonal rabbit antisera.
Lipopolysaccharide, NMR, DNA, Acinetobacter, Acinetobacter calcoaceticus, O-antigen structure, pyruvate
NCBI PubMed ID: 9030736Journal NLM ID: 0107600Publisher: Oxford, UK: Blackwell Science Ltd. on behalf of the Federation of European Biochemical Societies
Institutions: Division of Biochemical Microbiology, Center for Medicine and Biosciences, Research Center Borstel, Germany
Methods: NMR-2D, partial acid hydrolysis, NMR, composition analysis, GPC
Found