Taxonomic group: protista / Evosea
(Phylum: Evosea)
The structure was elucidated in this paperNCBI PubMed ID: 21979073Publication DOI: 10.1271/bbb.110369Journal NLM ID: 9205717Publisher: Japan Society for Bioscience, Biotechnology, and Agrochemistry
Correspondence: fujii
makino.kmu.ac.jp
Institutions: Laboratory of Chemistry, Kansai Medical University, Hirakata, Osaka, Japan, Department of Biophysics and Biochemistry, Osaka University Graduate School of Medicine, 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan
A secretory glycoprotein named Ψ-factor that we have purified and cloned from Dictyostelium discoideum is prespore cell-inducing factor. To address its functional significance, it is necessary to examine the attached sites and structures of its glycans as well as its protein structure. Here we identified and isolated a tryptic glycosylated peptide with the 71st to 89th amino acids of Ψ-factor that contained the consensus amino acid sequence for an N-linked glycan (N-T-T). MALDI-TOF mass spectrometry indicated that the major protonated molecular ions, [M+H](+), of the glycopeptide were present at m/z 3,806, the minor m/z 3,603 and 3,400 ions corresponding to the loss of one and two N-acetylhexosamines respectively. Digestion of it with N-glycosidase F gave a molecular mass of 1,766.9 for the whole glycan moiety, which accounts for its composition of five hexoses, four N-acetylhexosamines, and a deoxyhexose. Further digestion experiments on the basis of the substrate specificity of α-mannosidase and β-N-acetylhexosaminidase allowed us to elucidate the unique structure of the glycan, which contains a bisecting and an intersecting GlcNAc and a core α1,6-fucosyl moiety.
glycopeptide, bisecting-GlcNAc, Dictyostelium discoideum, intersecting-GlcNAc
Structure type: oligomer
Location inside paper: p. 1968, Fig. 6
Aglycon: Asn of gp3806
Trivial name: glycoprotein Ψ-factor
Compound class: N-glycan
Contained glycoepitopes: IEDB_123886,IEDB_130701,IEDB_135813,IEDB_136045,IEDB_137340,IEDB_137485,IEDB_140116,IEDB_141793,IEDB_141807,IEDB_141828,IEDB_142489,IEDB_144562,IEDB_144983,IEDB_151079,IEDB_151531,IEDB_152206,IEDB_152214,IEDB_153212,IEDB_153220,IEDB_164174,IEDB_174333,IEDB_187201,IEDB_429156,IEDB_548907,IEDB_857734,IEDB_983930,SB_197,SB_198,SB_33,SB_44,SB_67,SB_72,SB_73,SB_74,SB_77,SB_85,SB_86
Methods: SDS-PAGE, MALDI-TOF MS, enzymatic digestion, cloning, RP-HPLC, lectin blotting, protein assay
NCBI Taxonomy refs (TaxIDs): 44689
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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