Publication DOI:10.1186/s13071-017-2568-8 Journal NLM ID:101462774 Publisher: London: BioMed Central Correspondence: paulamn.biogmail.com Institutions: Instituto René Rachou/FIOCRUZ, Belo Horizonte, MG, Brazil, Departamento de Parasitologia, UFMG, Belo Horizonte, MG, Brazil
Background: Lipophosphoglycan (LPG) is a dominant surface molecule of Leishmania promastigotes. Its species-specific polymorphisms are found mainly in the sugars that branch off the conserved Gal(β1,4)Man(α1)-PO4 backbone of repeat units. Leishmania amazonensis is one of the most important species causing human cutaneous leishmaniasis in the New World. Here, we describe LPG intraspecific polymorphisms in two Le. amazonensis reference strains and their role during the development in three sand fly species. Results: Strains isolated from Lutzomyia flaviscutellata (PH8) and from a human patient (Josefa) displayed structural polymorphism in the LPG repeat units, possessing side chains with 1 and 2 β-glucose or 1 to 3 β-galactose, respectively. Both strains successfully infected permissive vectors Lutzomyia longipalpis and Lutzomyia migonei and could colonize their stomodeal valve and differentiate into metacyclic forms. Despite bearing terminal galactose residues on LPG, Josefa could not sustain infection in the restrictive vector Phlebotomus papatasi. Conclusions: LPG polymorphisms did not affect the ability of Le. amazonensis to develop late-stage infections in permissive vectors. However, the non-establishment of infection in Ph. papatasi by Josefa strain suggested other LPG-independent factors in this restrictive vector.
The structure was elucidated in this paper Publication DOI:10.1186/s13071-017-2568-8 Journal NLM ID:101462774 Publisher: London: BioMed Central Correspondence: paulamn.biogmail.com Institutions: Instituto René Rachou/FIOCRUZ, Belo Horizonte, MG, Brazil, Departamento de Parasitologia, UFMG, Belo Horizonte, MG, Brazil
Background: Lipophosphoglycan (LPG) is a dominant surface molecule of Leishmania promastigotes. Its species-specific polymorphisms are found mainly in the sugars that branch off the conserved Gal(β1,4)Man(α1)-PO4 backbone of repeat units. Leishmania amazonensis is one of the most important species causing human cutaneous leishmaniasis in the New World. Here, we describe LPG intraspecific polymorphisms in two Le. amazonensis reference strains and their role during the development in three sand fly species. Results: Strains isolated from Lutzomyia flaviscutellata (PH8) and from a human patient (Josefa) displayed structural polymorphism in the LPG repeat units, possessing side chains with 1 and 2 β-glucose or 1 to 3 β-galactose, respectively. Both strains successfully infected permissive vectors Lutzomyia longipalpis and Lutzomyia migonei and could colonize their stomodeal valve and differentiate into metacyclic forms. Despite bearing terminal galactose residues on LPG, Josefa could not sustain infection in the restrictive vector Phlebotomus papatasi. Conclusions: LPG polymorphisms did not affect the ability of Le. amazonensis to develop late-stage infections in permissive vectors. However, the non-establishment of infection in Ph. papatasi by Josefa strain suggested other LPG-independent factors in this restrictive vector.
Methods: acid hydrolysis, FACE, immunoblotting, CE, enzymatic treatment, macrophage activity assay, sand fly infections, morphometry Comments, role: the dephosphorylated repeat units from the PH8 strain consisted of a di- (67%), a tri- (30%) and a tetrasaccharide (3%).
The structure was elucidated in this paper Publication DOI:10.1186/s13071-017-2568-8 Journal NLM ID:101462774 Publisher: London: BioMed Central Correspondence: paulamn.biogmail.com Institutions: Instituto René Rachou/FIOCRUZ, Belo Horizonte, MG, Brazil, Departamento de Parasitologia, UFMG, Belo Horizonte, MG, Brazil
Background: Lipophosphoglycan (LPG) is a dominant surface molecule of Leishmania promastigotes. Its species-specific polymorphisms are found mainly in the sugars that branch off the conserved Gal(β1,4)Man(α1)-PO4 backbone of repeat units. Leishmania amazonensis is one of the most important species causing human cutaneous leishmaniasis in the New World. Here, we describe LPG intraspecific polymorphisms in two Le. amazonensis reference strains and their role during the development in three sand fly species. Results: Strains isolated from Lutzomyia flaviscutellata (PH8) and from a human patient (Josefa) displayed structural polymorphism in the LPG repeat units, possessing side chains with 1 and 2 β-glucose or 1 to 3 β-galactose, respectively. Both strains successfully infected permissive vectors Lutzomyia longipalpis and Lutzomyia migonei and could colonize their stomodeal valve and differentiate into metacyclic forms. Despite bearing terminal galactose residues on LPG, Josefa could not sustain infection in the restrictive vector Phlebotomus papatasi. Conclusions: LPG polymorphisms did not affect the ability of Le. amazonensis to develop late-stage infections in permissive vectors. However, the non-establishment of infection in Ph. papatasi by Josefa strain suggested other LPG-independent factors in this restrictive vector.
Methods: acid hydrolysis, FACE, immunoblotting, CE, enzymatic treatment, macrophage activity assay, sand fly infections, morphometry Comments, role: the dephosphorylated repeat units from the Josefa strain consisted of a di- (9%), a tri- (29%) and a tetrasaccharide (57%).
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