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1. (Article ID: 7372)
Cabib E, Blanco N, Arroyo J
Presence of a large β(1-3)glucan linked to chitin at the saccharomyces cerevisiae mother-bud neck suggests involvement in localized growth control
Eukaryotic Cell 11(4) (2012)
388-400
Previous results suggested that the chitin ring present at the yeast mother-bud neck, which is linked specifically to the nonreducing ends of β(1-3)glucan, may help to suppress cell wall growth at the neck by competing with β(1-6)glucan and thereby with mannoproteins for their attachment to the same sites. Here we explored whether the linkage of chitin to β(1-3)glucan may also prevent the remodeling of this polysaccharide that would be necessary for cell wall growth. By a novel mild procedure, β(1-3)glucan was isolated from cell walls, solubilized by carboxymethylation, and fractionated by size exclusion chromatography, giving rise to a very high-molecular-weight peak and to highly polydisperse material. The latter material, soluble in alkali, may correspond to glucan being remodeled, whereas the large-size fraction would be the final cross-linked structural product. In fact, the β(1-3)glucan of buds, where growth occurs, is solubilized by alkali. A gas1 mutant with an expected defect in glucan elongation showed a large increase in the polydisperse fraction. By a procedure involving sodium hydroxide treatment, carboxymethylation, fractionation by affinity chromatography on wheat germ agglutinin-agarose, and fractionation by size chromatography on Sephacryl columns, it was shown that the β(1-3)glucan attached to chitin consists mostly of high-molecular-weight material. Therefore, it appears that linkage to chitin results in a polysaccharide that cannot be further remodeled and does not contribute to growth at the neck. In the course of these experiments, the new finding was made that part of the chitin forms a noncovalent complex with β(1-3)glucan.
protein, glucan, solvent, glycosidase, Saccharomyces cerevisiae, chitin, glucan endo 1, 3 beta glucosidase, sodium hydroxide
NCBI PubMed ID: 22366124Publication DOI: 10.1128/EC.05328-11Journal NLM ID: 101130731Publisher: American Society for Microbiology
Correspondence: enricoc
bdg10.niddk.nih.gov
Institutions: Laboratory of Biochemistry and Genetics, NIDDK, National Institutes of Health, Department of Health and Human Services, Bethesda, USA, Departamento de Microbiología II, Facultad de Farmacia, Universidad Complutense de Madrid, Madrid, Spain, Laboratory of Biochemistry and Genetics, NIDDK, National Institutes of Health, Department of Health and Human Services, Bethesda, United States
Methods: methylation, alkaline hydrolysis, CC
The publication contains the following compound(s):
- Compound ID: 15621
Structure type: fragment of a bigger structure
Trivial name: pustulan, 1-6-β-glucan, alkali-soluble β-glucan (PeA3)
Compound class: EPS, O-polysaccharide, cell wall polysaccharide, glucan, b-glucan
Reference(s) to other database(s): GTC:G26777BZ
- Compound ID: 18385
Structure type: homopolymer
; n=1500
Trivial name: β-glucan, 1-3-β-glucan, curdlan
Compound class: O-polysaccharide, cell wall polysaccharide, D-glucan
Reference(s) to other database(s): GTC:G51056AN
- Compound ID: 18741
Structure type: structural motif or average structure
; n=1500
Trivial name: CM-β-(1-3)-glucan
Compound class: glucan
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