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1. (Article ID: 7375)
Dey B, Bhunia SK, Maity KK, Patra S, Mandal S, Maiti S, Maiti TK, Sikdar SR, Islam SS
Glucans of Pleurotus florida blue variant: isolation, purification, characterization and immunological studies
International Journal of Biological Macromolecules 50(3) (2012)
591-597
Two different glucans (PS-I and PS-II) were isolated from the alkaline extract of the fruiting bodies of an edible mushroom, Pleurotus florida blue variant and the PS-I showed macrophage, splenocyte and thymocyte activations. On the basis of sugar analysis, methylation analysis, periodate oxidation, and NMR studies (1H, 13C, DEPT-135, DQF-COSY, TOCSY, NOESY, ROESY, HMQC and HMBC), the structure of the repeating unit of these polysaccharides were established.
structure, NMR spectroscopy, mushroom polysaccharide, Immunoactivation, Pleurotus florida blue variant
NCBI PubMed ID: 22322111Publication DOI: 10.1016/j.ijbiomac.2012.01.031Journal NLM ID: 7909578Publisher: Butterworth-Heinemann
Correspondence: Islam SS
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Institutions: Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore, India, Division of Plant Biology, Bose Institute, Kolkata, India, Department of Biotechnology, Indian Institute of Technology (IIT) Kharagpur, Kharagpur, India
Methods: 13C NMR, 1H NMR, methylation, periodate oxidation, GLC-MS, NMR-2D, GLC, paper chromatography, extraction, gel chromatography, optical rotation measurement, generation of reactive oxygen species (ROS), cytokine production, DEPT, MTT, splenocyte proliferation assay, thymocyte proliferation assay
The publication contains the following compound(s):
- Compound ID: 18745
Structure type: homopolymer
; 202000
Trivial name: 1-6-β-glucan
Compound class: O-polysaccharide, cell wall polysaccharide, D-glucan
Reference(s) to other database(s): GTC:G26777BZ
- Compound ID: 18746
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a-D-Glcp-(1-4)-+
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-3)-b-D-Glcp-(1-3)-b-D-Glcp-(1-3)-b-D-Glcp-(1-3)-a-D-Glcp-(1- |
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Structure type: polymer chemical repeating unit
; 176000
Trivial name: (1-3,4)-glucan, pleurotan
Compound class: O-polysaccharide, cell wall polysaccharide, D-glucan
Reference(s) to other database(s): GTC:G21016AK
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