Found 1 publication.
Displayed publication 1
Expand all publications
Collapse all publications
Show all as text (SweetDB notation)
Show all graphically (SNFG notation)
1. (Article ID: 7378)
Bardalaye PC, Nordin JH
Chemical structure of the galactomannan from the cell wall of Aspergillus niger
Journal of Biological Chemistry 252(8) (1977)
2584-2591
The galactomannan of surface grown Aspergillus niger has been isolated by alkaline extraction of hyphal walls and characterized structurally. Its elution profile, from a column of Bio-Gel P-150, reveals a broad range of molecular sizes grouped into two fractions. Gas chromatographic and calorimetric analyses indicate that each fraction is composed of approximately equimolar quantities of galactose and mannose plus 12 to 14% glucose. Both have similar low optical rotations and contain acid labile galactose. Methylation, Smith degradation, acetolysis, reactivity with concanavalin A and β-D-galactofuranosidase, plus digestion of galactose with D-galactose oxidase, were techniques employed to determine the polysaccharide's covalent structure. Results of these studies indicate that it is composed of a series of chains, 5 to 9 hexose units in length, connected by α1→6 bonds between mannopyranosyl moieties. The external portion of each chain consists of a galactose tri- or tetrasaccharide of the general structure Galf β1→4 Galp(one or two) 1→4 Galp l→. This segment is connected via a (1→2) linkage to the internal portion which is a di- to pentasaccharide of mannopyranosyl units joined in α(1→2) glycoside linkage. Combination mild acid hydrolysis and methylation experiments indicate galactofuranosyl terminal units are attached only to galactose. The organization of glucose into the overall structure of the polymer has not been determined. Structural relationships of this polysaccharide to both fungal and yeast galactomannans are discussed.
cell wall, polysaccharides, glucose, galactose, plant, Mannose, Galactomannan, molecular weight, Molecular Conformation, glycerol, erythritol, microorganism, Aspergillus, Mannans, Aspergillus niger, in vitro study, theoretical study
NCBI PubMed ID: 856795Journal NLM ID: 2985121RPublisher: Baltimore, MD: American Society for Biochemistry and Molecular Biology
Institutions: Department of Biochemistry, University of Massachusetts, Amherst, USA
Methods: methylation, periodate oxidation, TLC, acid hydrolysis, GC, Smith degradation, paper chromatography, GPC, enzymatic digestion, extraction, optical rotation measurement, acetolysis, NaBH4 reduction, reduction, phenol-sulfuric acid assay, quantitative precipitation assay, phosphate measurement
The publication contains the following compound(s):
- Compound ID: 18749
|
b-D-Galf-(1-4)-b-D-Galp-(1-4)-b-D-Galp-(1-4)-b-D-Galp-(1-2)-+
|
b-D-Galf-(1-4)-b-D-Galp-(1-4)-b-D-Galp-(1-4)-b-D-Galp-(1-2)-+ |
| |
b-D-Galf-(1-4)-b-D-Galp-(1-4)-b-D-Galp-(1-2)-+ | | b-D-Galf-(1-4)-b-D-Galp-(1-4)-b-D-Galp-(1-2)-+
| | | |
-2)-a-D-Manp-(1-6)-a-D-Manp-(1-2)-a-D-Manp-(1-2)-a-D-Manp-(1-2)-a-D-Manp-(1-6)-a-D-Manp-(1-2)-a-D-Manp-(1-6)-a-D-Manp-(1-2)-a-D-Manp-(1-2)-a-D-Manp-(1-2)-a-D-Manp-(1-6)-a-D-Manp-(1-2)-a-D-Manp-(1-2)-a-D-Manp-(1- |
Show graphically |
Structure type: structural motif or average structure
Compound class: O-polysaccharide, galactomannan
Reference(s) to other database(s): GTC:G64142SF
- Compound ID: 18750
|
b-D-Galp-(1-2)-+
|
-6)-a-D-Manp-(1-2)-a-D-Manp-(1-2)-a-D-Manp-(1- |
Show graphically |
Structure type: fragment of a bigger structure
Compound class: O-polysaccharide, galactomannan
Reference(s) to other database(s): GTC:G41468ZO
- Compound ID: 18751
|
b-D-Galp-(1-2)-a-D-Manp-(1-2)-+
|
-6)-a-D-Manp-(1-2)-a-D-Manp-(1- |
Show graphically |
Structure type: fragment of a bigger structure
Compound class: O-polysaccharide, galactomannan
Reference(s) to other database(s): GTC:G80284TP
- Compound ID: 18752
|
b-D-Galp-(1-2)-a-D-Manp-(1-2)-a-D-Manp-(1-2)-+
|
-6)-a-D-Manp-(1- |
Show graphically |
Structure type: fragment of a bigger structure
Compound class: O-polysaccharide, galactomannan
Reference(s) to other database(s): GTC:G31471VB
Expand this publication
Collapse this publication
Total list of publication IDs on all result pages of the current query:
Total list of corresponding CSDB IDs (permanent record IDs):
Execution: 1 sec