A polysaccharide containing the residues of 2-keto-3-deoxy-D-glycero-D-galacto-nonulosonic acid (Kdn) was found in the cell wall of the Brevibacterium casei strain AEI Ac-2114T . The polymer structure was elucidated by analyzing one-dimensional spectra of 1H and 13C NMR and bidimentional experiments 1H/13C-COSY, TOCSY, 1H/13C-gHSQC, and 1H/13C-gHMBC. The polymer is built up of the 2→4-linked Kdn residues substituted by β-D-Glcp residues at 8- and 9-hydroxyls; such a polymer with disubstituted Kdn residues was found for the first time. A glycosylated teichoic acid of the 1,3-poly(glycerophosphate) type was also identified among other anionic polymers of cell wall.
NMR spectroscopy, teichoic acids, Kdn, Brevibacterium, 3-deoxy-D-glycero-D-galactonon-2-ulosonic acid
NCBI PubMed ID: 17375662Journal NLM ID: 9420101Publisher: Springer Science and Business Media
Correspondence: potekhina@hotbox.ru
Institutions: Biological Faculty, Moscow State University, Vorob’evy gory, Moscow, 119992 Russia,All-Russian Collection of Microorganisms, Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Moscow oblast, 142292 Russia,Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia
Methods: 13C NMR, 1H NMR, NMR-2D, sugar analysis, acid hydrolysis, GLC, NMR-1D
Found