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1. (Article ID: 7446)
Smiderle FR, Sassaki GL, van Arkel J, Iacomini M, Wichers HJ, van Griensven LJ
High molecular weight glucan of the culinary medicinal mushroom Agaricus bisporus is an α-glucan that forms complexes with low molecular weight galactan
Molecules 15(8) (2010)
5818-5830
An α-glucan was isolated from the culinary medicinal mushroom A. bisporus by hot water extraction, ethanol precipitation and DEAE-cellulose chromatography. The resulting material showed a single HMW peak excluded from a Sephadex G50 column that could completely be degraded by α-amylase treatment. After heating in 1% SDS a small additional peak of low MW eluted from the G50 column. The monosaccharide composition of the main peak was evaluated by HPLC, and was found to consist of a majority of glucose (97.6%), and a minor proportion of galactose (2.4%). Methylation analysis and degradation by α-amylase indicated the presence of an α-glucan with a main chain consisting of (1→4)-linked units, substituted at O-6 by α-D-glucopyranose single-units in the relation 1:8. Mono- ( 13C-, 1H-NMR) and bidimensional [1H (obs.),13C-HSQC] spectroscopy analysis confirmed the α-configuration of the Glcp residues by low frequency resonances of C-1 at δ 100.6, 100.2, and 98.8 ppm and H-1 high field ones at δ 5.06, 5.11, and 4.74 ppm. The DEPT-13C-NMR allowed assigning the non-substituted and Osubstituted -CH2 signals at δ 60.3/60.8 and 66.2 ppm, respectively. Other assignmentswere attributed to C-2, C-3, C-4, C-5 and C-6 of the non-reducing ends at δ 71.8; 72.8; 70.0; 71.3 and 60.3/60.8 ppm, respectively. The minor proportion of galactose that was demonstrated was probably derived from a complex between the α-glucan and a low molecular weight galactan.
NMR, polysaccharides, α-glucan, Basidiomycetes, medicinal mushrooms, Agaricus bisporus
NCBI PubMed ID: 20736909Publication DOI: 10.3390/molecules15085818Journal NLM ID: 100964009Publisher: Basel, Switzerland: MDPI
Correspondence: leo.vangriensven
wur.nl
Institutions: Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, Curitiba, Brazil, Plant Research International, Wageningen University and Research Centre, Wageningen, The Netherlands, Wageningen University and Research Centre, Wageningen, The Netherlands
Methods: 13C NMR, 1H NMR, GC-MS, acid hydrolysis, HPLC, enzymatic digestion, ion-exchange chromatography, extraction, SEC, reduction, phenol-sulfuric acid assay, ethanol precipitation, HSQC
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