Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Escherichia coli [ICD11:
XN6P4 
]
Journal NLM ID: 8008051Publisher: Kluwer Academic Publishers
Correspondence: matilde.manzoni
unimi.it
Institutions: Dipartimento di Scienze e Tecnologie Alimentari e Microbiologiche, Sezione di Microbiologia Industriale, Universit`a degli Studi, Via G. Celoria 2, 20133 Milano, Italia, PALL Italia, via G. Bruzzesi 38/40, 20146 Milano, Italy.
The K5 polysaccharidic antigen obtainable from a strain of Escherichia coli is the non-sulphated precursor in heparin biosynthesis; K5 is composed by two components, 16 000 and 1500 Da, their ratio depending on the fermentation conditions. In this study an assessment was made of how various cell-culture filtrate separation technologies affected the components themselves and their ratio. A dynamic membrane filtration technology, a tubular ceramic module for tangential flow microfiltration and the standard discontinuous centrifugation were comparatively employed to perform the separation. Experiments carried out on E. coli cultures containing the two components in different ratios showed that the DMF system is a suitable technology for K5 isolation.
antigen, capsular polysaccharide, cell, Escherichia coli, extracellular, dynamic microfiltration technology, fermentation, recovery, separation
Structure type: polymer chemical repeating unit ; 16000, 1500
Location inside paper: p.759
Compound class: CPS
Contained glycoepitopes: IEDB_115136,IEDB_140630,IEDB_141807,IEDB_151531,IEDB_153764,IEDB_423153
Methods: dynamic membrane filtration
NCBI Taxonomy refs (TaxIDs): 562Reference(s) to other database(s): GTC:G26089XS, GlycomeDB:
656
Show glycosyltransferases
There is only one chemically distinct structure:
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