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1. (Article ID: 7520)
Akao T, Yahara A, Sakamoto K, Yamada O, Akita O, Yoshida T
Lack of endoplasmic reticulum 1,2-α-mannosidase activity that trims N-glycan Man9GlcNAc2 to Man8GlcNAc2 isomer B in a manE gene disruptant of Aspergillus oryzae
Journal of Bioscience and Bioengineering 113(4) (2012)
438-441
The gene manE, encoding a probable class I endoplasmic reticulum 1,2-α-mannosidases (ER-Man), was identified from the filamentous fungus Aspergillus oryzae due to similarity to orthologs. It removes a single mannose residue from Man9GlcNAc2, generating Man8GlcNAc2 isomer B. Disruption of manE caused drastic decreases in ER-Man activity in A. oryzae microsomes.
glycoprotein, 1, N-glycan processing, endoplasmic reticulum, Aspergillus oryzae, 2-α-mannosidase
NCBI PubMed ID: 22169093Publication DOI: 10.1016/j.jbiosc.2011.11.015Journal NLM ID: 100888800Publisher: Osaka, Japan, Amsterdam, The Netherlands: Society for Bioscience and Bioengineering
Correspondence: akao_t
nrib.go.jp
Institutions: National Research Institute of Brewing, Hiroshima, Japan, Faculty of Agriculture and Life Science, Hirosaki University, Hirosaki, Japan
Methods: DNA sequencing, TLC, HPLC, fluorescence spectroscopy, cell growth
The publication contains the following compound(s):
- Compound ID: 13270
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a-D-Manp-(1-2)-a-D-Manp-(1-2)-a-D-Manp-(1-3)-+
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a-D-Manp-(1-2)-a-D-Manp-(1-6)-+ |
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a-D-Manp-(1-2)-a-D-Manp-(1-3)-a-D-Manp-(1-6)-b-D-Manp-(1-4)-b-D-GlcpNAc-(1-4)-b-D-GlcpNAc |
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Structure type: oligomer
Trivial name: N-linked oligosaccharide
Compound class: N-glycan, glycoprotein, polysaccharide, mannan, N-polysaccharide
Reference(s) to other database(s): GTC:G60230HH
- Compound ID: 18475
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a-D-Manp-(1-2)-a-D-Manp-(1-2)-a-D-Manp-(1-3)-+
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a-D-Manp-(1-3)-+ |
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a-D-Manp-(1-2)-a-D-Manp-(1-6)-a-D-Manp-(1-6)-b-D-Manp-(1-4)-b-D-GlcpNAc-(1-4)-b-D-GlcpNAc |
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Structure type: oligomer
Compound class: N-glycan
Reference(s) to other database(s): GTC:G40702WU
- Compound ID: 19064
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a-D-Manp-(1-2)-a-D-Manp-(1-3)-+
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a-D-Manp-(1-2)-a-D-Manp-(1-6)-+ |
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a-D-Manp-(1-2)-a-D-Manp-(1-3)-a-D-Manp-(1-6)-b-D-Manp-(1-4)-b-D-GlcpNAc-(1-4)-b-D-GlcpNAc |
Show graphically |
Structure type: oligomer
Compound class: polysaccharide, N-polysaccharide
Reference(s) to other database(s): GTC:G89864BN
- Compound ID: 19065
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a-D-Manp-(1-2)-a-D-Manp-(1-2)-a-D-Manp-(1-3)-+
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a-D-Manp-(1-6)-+ |
| |
a-D-Manp-(1-2)-a-D-Manp-(1-3)-a-D-Manp-(1-6)-b-D-Manp-(1-4)-b-D-GlcpNAc-(1-4)-b-D-GlcpNAc |
Show graphically |
Structure type: oligomer
Reference(s) to other database(s): GTC:G91704UR
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