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1. (Article ID: 7525)
 
Johnston IR
The partial acid hydrolysis of a highly dextrorotatory fragment of the cell wall of Aspergillus niger. Isolation of the α-(1→3)-linked dextrin series
Biochemical Journal 96(3) (1965) 659-664
 

1. A highly dextrorotatory polysaccharide (α(D)+232° in N-sodium hydroxide), previously isolated as a fragment of Aspergillus niger cell walls, was prepared from whole mycelium and subjected to partial acid hydrolysis. 2. Fractionation of the hydrolysate on a charcoal column with a linear gradient of ethanol yielded a series of oligosaccharides. The disaccharide member was shown to be nigerose (3-O-α-d-glucopyranosyl-α-d-glucopyranose), although a small proportion of the disaccharide peak (10%) was present as maltose despite the fact that all the nigeran had been removed from the starting material. 3. The oligosaccharides forming the main peaks from the column were shown to be members of a polymer-homologous series (nigerodextrins) by (a) the relationship between the logarithm of their chromatographic mobility and degree of polymerization, (b) obeying the Freudenberg relationship, and (c) partial acid hydrolysis.

cell wall, Aspergillus niger, nigeran

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