A polysaccharide containing D-Manp, L-Fucp (6-deoxygalactopyranose, fucose) and D-GlcpNAc was isolated by mild acid hydrolysis, followed by gel-permeation chromatography, from the lipopolysaccharide derived from Acinetobacter strain 96 (DNA group 11). The structure of the O-antigen was determined by compositional analysis and NMR spectroscopy of the polysaccharide as: [carbohydrate structure see text] A monoclonal antibody obtained after immunization of mice with heat-killed bacteria of Acinetobacter strain 96 was shown to bind to the O-antigen and did not cross-react with any Acinetobacter O-antigen of known structure.
Lipopolysaccharide, NMR, antigen, structure, blotting, chemistry, Bacterial, human, DNA, strain, structural, polysaccharide, O-antigen, analysis, O antigen, group, O-antigenic, O-antigenic polysaccharide, acid, Acinetobacter, antibodies, antibody, epitope, monoclonal, monoclonal antibodies, monoclonal antibody, animal, antigens, Carbohydrate Sequence, immunization, immunology, mice, Inbred BALB C, Molecular Sequence Data, epitopes, NMR spectroscopy, Cross Reactions, O antigens, O-antigens, bacteria, hydrolysis, serological, biological, Magnetic Resonance Spectroscopy, spectroscopy, gel permeation chromatography, Western, Carbohydrate Conformation, chromatography, classification, fucose, serotyping
NCBI PubMed ID: 11521091Journal NLM ID: 9433350Publisher: Maney Publishing
Correspondence: oholst@fzborstel.de
Institutions: Institute for Biological Sciences, NRCC, Ottawa, Ontario, Canada, The Scripps Research Institute, Department of Immunology, La Jolla, California, USA, Divisions of Medical and Biochemical Microbiology, Analytical Biochemistry, Research Center Borstel, Borstel, Germany
Methods: NMR-2D, NMR, composition analysis
Found