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1. (Article ID: 770)
 
Chouly C, Colquhoun IJ, Jodelet A, York G, Walker GC
NMR studies of succinoglycan repeating-unit octasaccharides from Rhizobium meliloti and Agrobacterium radiobacter
International Journal of Biological Macromolecules 17 (1995) 357-363
 

Complete 1H and 13C-nuclear magnetic resonance assignments have been obtained for the octasaccharide repeating units of the bacterial polysaccharide succinoglycan from Rhizobium meliloti Rm1021 and Agrobacterium radiobacter NCIB 11883. The assignments were used to determine the locations of the O-succinyl and O-acetyl substituents. The O-acetyl substituent in Rm1021 was attached to the 3rd residue from the reducing end, and the O-succinyl group was attached to the 7th residue in both octasaccharides. The structure of the Rm1021 octasaccharide is as shown below: [formula: see text] A small amount of succinate was also attached to C6 of the 6th residue in both octasaccharides.

NMR, structure, polysaccharide, repeating unit, Rhizobia, Rhizobium, extracellular, Rhizobium meliloti, Agrobacterium, Agrobacterium radiobacter, succinoglycan

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