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1. (Article ID: 7724)
Ning Y, Chen F, Xu X, Jin Y, Wang Z, Yang K, Jia Y
Biosynthesis of neokestose laurate catalyzed by Candida antarctica lipase B and its antimicrobial activity against food pathogenic and spoilage bacteria
Journal of Agricultural and Food Chemistry 65(50) (2017)
11092-11099
To increase the functionality and broaden the potential application of neokestose, neokestose laurate was biosynthesized using Candida antarctica lipase B as biocatalyst, for which a mixture of 20% DMSO in 2-methyl-2-butanol (v/v) was chosen as the reaction medium. The optimum conditions for biosynthesis were as follows: a molar ratio of vinyl laurate to neokestose of 12, a temperature of 50 °C, molecular sieves of 100 g/L, and enzyme loading of 10 g/L. Under the optimal conditions, the conversion rate was achieved over 80%. The synthesized chemical 6'-O-lauroylneokestose confirmed by nuclear magnetic resonance (NMR) exhibited good emulsification with critical micelle concentration (CMC) of 352 μM and broad antibacterial activity against Gram-positive bacteria such as Staphylococcus aureus, Listeria monocytogenes, Streptococcus mutans, Bacillus subtilis, and Bacillus cereus. Conclusively, 6'-O-lauroylneokestose was evidenced to be a dual-functional agent with emulsification and antibacterial activity, showing promising application potential in the food industry.
acylation, antimicrobial activity, Candida antarctica lipase B, neokestose, neokestose laurate
NCBI PubMed ID: 29185745Publication DOI: 10.1021/acs.jafc.7b04608Journal NLM ID: 0374755Publisher: American Chemical Society
Correspondence: jiayingmin
btbu.edu.cn
Institutions: School of Food Science and Technology, Jiangnan University, Wuxi, China, School of Biological Engineering, Hebei University of Science and Technology, Shijiazhuang, China, Department of Food, Nutrition and Packaging Sciences, Clemson University, Clemson, USA, Department of Chemistry, Clemson University, Clemson, USA, School of Food and Chemical Engineering, Beijing Technology and Business University, Beijing, China
Methods: 13C NMR, 1H NMR, ESI-MS, HPLC, HMBC, COSY, HSQC
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