Two glucans, one α-glucan (TM-I) and one β-glucan (TM-II) were isolated from an edible mushroom, Termitomyces microcarpus by extraction with 5% NaOH/0.05% NaBH4, followed by a precipitation with 1 M acetic acid. The repeating units of two polysaccharides have been identified by means of NMR studies (1H, 13C, DEPT-135 and HSQC) and chemical investigations. The structures of repeating units of two polymers were established.

polysaccharide, mushroom, glucan, HSQC, Termitomyces microcarpus

Publication DOI: 10.1177/1934578X1701201226
Journal NLM ID: 101477873
Publisher: Natural Product Communications
Correspondence: diliprout.chemgmail.com
Institutions: Department of Chemistry, Government General Degree College, Kharagpur, India, Department of Chemistry, Jhargram Raj College, Jhargram, India
Methods: 13C NMR, 1H NMR, periodate oxidation, GLC-MS, acetylation, methylation analysis, reduction with NaBH4, HSQC, DEPT-135, TFA hydrolysis
 

• Compound ID: 13319
  

  -3)-a-D-Glcp-(1-

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  Structure type: homopolymer
  Trivial name: D-rhamnan, α-1,3-D-glucan, α-1,3-glucan, (1-3)-α-Glucan, 1-3-α-glucan, α-(1,3)-glucan, α-(1,3)glucan, (1-3)-α-glucan, pseudonigeran, α-(1,3)-glucan, pseudonigeran, water-insoluble α-D-glucan (TM-I)
  Compound class: EPS, O-polysaccharide, cell wall polysaccharide, glucan, polysaccharide, D-glucan
  Reference(s) to other database(s): GTC:G02177KX, CCSD:49417, CBank-STR:17683, GenDB:HM245773
  
   
  
  Termitomyces microcarpus
  CSDB ID 44600 (all data & tools)
• Compound ID: 17106
  

  b-D-Glcp-(1-6)-+ | -3)-b-D-Glcp-(1-3)-b-D-Glcp-(1-

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  Structure type: polymer chemical repeating unit
  Trivial name: lentinan, water-insoluble β-D-glucan (TM-II)
  Compound class: EPS, glucan, polysaccharide
  Reference(s) to other database(s): GTC:G70326NL, CCSD:9766, CBank-STR:7239
  
   
  
  Termitomyces microcarpus
  CSDB ID 44601 (all data & tools)