Found 1 publication. Displayed publication 1
Expand all publications       Show all as text (SweetDB notation)
1. (Article ID: 7771)
 
Dong Y, Teng H, Qi S, Liu L, Wang H, Zhao Y, Xiu Z
Pathways and kinetics analysis of biotransformation of Dioscorea zingiberensis by Aspergillus oryzae
Biochemical Engineering Journal 52 (2010) 123–130
 

In this paper, the pathways and kinetics for the production of diosgenin via biotransformation of Dioscorea zingiberensis C.H. Wright by Aspergillus oryzae CICC 2436 were analyzed. After 120 h of biotransformation at 30 ◦C, the concentration of diosgenin in the culture reached 36.87±1.27 μmol/g raw herb, which was 21.2 times its initial concentration. A number of steroidal compounds were also isolated as minor products from the biotransformation, and one of these was identified as a novel compound named 3-O-β-D-glucopyranosyl (1→3) – β-D-glucopyranosyl (1→4) – β-D-glucopyranosyl-diosgenin (diosgenin-triglucoside). The biotransformation consisted of two stages: the release of steroids from the herb (accompanied by fungal growth) and hydrolysis of the steroids by glycosidases. Kinetic analysis and mathematical modelling showed that the process of biotransformation could be described by first order kinetics under the condition of high Km/[S] values. It consisted of a cascade of consecutive and parallel reactions involving three kinds of enzymes, five steroid saponins and their sapogenin. The main hydrolysis reactions that led to the production of diosgenin were also discussed.

biotransformation, Aspergillus oryzae, Dioscorea zingiberensis, diosgenin, pathways analysis, kinetic modeling

The publication contains the following compound(s):
 

Expand this publication

Resort publications by:

New query Export IDs Home Help

Execution: <1 sec