Extraction of dry bacteria of Acinetobacter baumannii strain 24 by phenol-water yielded a lipopolysaccharide (LPS) that was studied by serological methods and fatty acid analysis. After immunisation of BALB/c mice with this strain, monoclonal antibody S48-3-13 (IgG(3) isotype) was obtained, which reacted with the LPS in western blot and characterized it as S-form LPS. Degradation of the LPS in aqueous 1% acetic acid followed by GPC gave the O-antigenic polysaccharide, whose structure was determined by compositional analyses and NMR spectroscopy of the polysaccharide and O-deacylated polysaccharide as [carbohydrate structure: see text] where QuiN4N is 2,4-diamino-2,4,6-trideoxyglucose and GalNAcA 2-acetamido-2-deoxygalacturonic acid. The amino group at C-4 of the QuipN4N residues is acetylated in about 2/3 of LPS molecules and (S)-3-hydroxybutyrylated in the rest
Lipopolysaccharide, NMR, LPS, structure, strain, structural, polysaccharide, analysis, group, molecule, O-antigenic, O-antigenic polysaccharide, acid, Acinetobacter, Acinetobacter baumannii, antibodies, antibody, monoclonal, monoclonal antibodies, monoclonal antibody, mice, NMR spectroscopy, bacteria, serological, spectroscopy, fatty acid, method, degradation, methods, acetylated, amino, amino group, aqueous, extraction, O-deacylated, phenol-water, S-form
NCBI PubMed ID: 14670733Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: oholst@fz-borstel.de
Institutions: Division of Medical and Biochemical Microbiology, Research Center Borstel, D-23845 Borstel, Germany, Division of Structural Biochemistry, Research Center Borstel, D-23845 Borstel, Germany
Methods: NMR-2D, NMR, chemical methods
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