Casillo A, Fabozzi A, Russo Krauss I, Parrilli E, Biggs CI, Gibson MI, Lanzetta R, Arato V, Appavou MS, Radulescu A, Tutino ML, Paduano L, Corsaro MM Physicochemical Approach to Understanding the Structure, Conformation, and Activity of Mannan Polysaccharides Biomacromolecules22(4) (2021)
1445-1457
The structure was elucidated in this paper NCBI PubMed ID:33729771 Publication DOI:10.1021/acs.biomac.0c01659 Journal NLM ID:100892849 Publisher: Washington, DC: American Chemical Society Correspondence: Luigi Paduano <lpaduanounina.it>; Maria M. Corsaro <corsarounina.it> Institutions: Department of Chemical Sciences, University of Naples 'Federico II', Complesso Universitario Monte S. Angelo, Via Cintia 4, 80126 Naples, Italy, CSGI - Consorzio per lo Sviluppo dei Sistemi a Grande Interfase, Florence, Italy, Department of Chemistry, University of Warwick, Coventry CV4 7AL, U.K, Jülich Centre for Neutron Science, Garching Forschungszentrum, Lichtenbergstrasse 1, D-857478 Garching bei Munchen, Germany
Extracellular polysaccharides are widely produced by bacteria, yeasts, and algae. These polymers are involved in several biological functions, such as bacteria adhesion to surface and biofilm formation, ion sequestering, protection from desiccation, and cryoprotection. The chemical characterization of these polymers is the starting point for obtaining relationships between their structures and their various functions. While this fundamental correlation is well reported and studied for the proteins, for the polysaccharides, this relationship is less intuitive. In this paper, we elucidate the chemical structure and conformational studies of a mannan exopolysaccharide from the permafrost isolated bacterium Psychrobacter arcticus strain 273-4. The mannan from the cold-adapted bacterium was compared with its dephosphorylated derivative and the commercial product from Saccharomyces cerevisiae. Starting from the chemical structure, we explored a new approach to deepen the study of the structure/activity relationship. A pool of physicochemical techniques, ranging from small-angle neutron scattering (SANS) and dynamic and static light scattering (DLS and SLS, respectively) to circular dichroism (CD) and cryo-transmission electron microscopy (cryo-TEM), have been used. Finally, the ice recrystallization inhibition activity of the polysaccharides was explored. The experimental evidence suggests that the mannan exopolysaccharide from P. arcticus bacterium has an efficient interaction with the water molecules, and it is structurally characterized by rigid-rod regions assuming a 14-helix-type conformation.
Casillo A, Fabozzi A, Russo Krauss I, Parrilli E, Biggs CI, Gibson MI, Lanzetta R, Arato V, Appavou MS, Radulescu A, Tutino ML, Paduano L, Corsaro MM Physicochemical Approach to Understanding the Structure, Conformation, and Activity of Mannan Polysaccharides Biomacromolecules22(4) (2021)
1445-1457
The structure was elucidated in this paper NCBI PubMed ID:33729771 Publication DOI:10.1021/acs.biomac.0c01659 Journal NLM ID:100892849 Publisher: Washington, DC: American Chemical Society Correspondence: Luigi Paduano <lpaduanounina.it>; Maria M. Corsaro <corsarounina.it> Institutions: Department of Chemical Sciences, University of Naples 'Federico II', Complesso Universitario Monte S. Angelo, Via Cintia 4, 80126 Naples, Italy, CSGI - Consorzio per lo Sviluppo dei Sistemi a Grande Interfase, Florence, Italy, Department of Chemistry, University of Warwick, Coventry CV4 7AL, U.K, Jülich Centre for Neutron Science, Garching Forschungszentrum, Lichtenbergstrasse 1, D-857478 Garching bei Munchen, Germany
Extracellular polysaccharides are widely produced by bacteria, yeasts, and algae. These polymers are involved in several biological functions, such as bacteria adhesion to surface and biofilm formation, ion sequestering, protection from desiccation, and cryoprotection. The chemical characterization of these polymers is the starting point for obtaining relationships between their structures and their various functions. While this fundamental correlation is well reported and studied for the proteins, for the polysaccharides, this relationship is less intuitive. In this paper, we elucidate the chemical structure and conformational studies of a mannan exopolysaccharide from the permafrost isolated bacterium Psychrobacter arcticus strain 273-4. The mannan from the cold-adapted bacterium was compared with its dephosphorylated derivative and the commercial product from Saccharomyces cerevisiae. Starting from the chemical structure, we explored a new approach to deepen the study of the structure/activity relationship. A pool of physicochemical techniques, ranging from small-angle neutron scattering (SANS) and dynamic and static light scattering (DLS and SLS, respectively) to circular dichroism (CD) and cryo-transmission electron microscopy (cryo-TEM), have been used. Finally, the ice recrystallization inhibition activity of the polysaccharides was explored. The experimental evidence suggests that the mannan exopolysaccharide from P. arcticus bacterium has an efficient interaction with the water molecules, and it is structurally characterized by rigid-rod regions assuming a 14-helix-type conformation.
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unina.it>; Maria M. Corsaro <corsaro