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1. (Article ID: 799)
Datta AK, Basu S
Structure of the O-specific side chain of the non-capsulated Escherichia coli O125 lipopolysaccharide
Biochemical Archives 14(2) (1998)
131-138
The O-specific polysaccharide isolated from the non-capsulated Escherichia coli O125: K-:H19 lipopolysaccharide contains L-fucose, D-mannose, D-galactose, D-glucos and 2-acetamido-2-deoxy-D-galactose in the molar retios 1:1:1:1:1.7. The primary structure of the O-specific polysaccharide was established by compositional analysis, methylation studies, enzymatic hydrolysis and with the 1H and 13C NMR spectroscopy. These studies revealed that the O-antigen is branched and built up of repeating hexasaccharide unit having th following structure: (see struc. in text). This structure is similar to that of recently reported during the course of our investigation for the O-antigenic polysaccharide for a capsulated strain of Escherichia coli O125:K72:H19 of European origin.
Lipopolysaccharide, structure, chain, side chain, Escherichia, Escherichia coli, O-specific
Journal NLM ID: 8705043Publisher: M.B.R. Press
Institutions: Department of Biological Chemistry, Indian Association for the Cultivation of Sciences, Calcutta, India
Methods: 13C NMR, 1H NMR, methylation, sugar analysis, enzymatic hydrolysis, Smith degradation
The publication contains the following compound(s):
- Compound ID: 2259
|
b-D-Galp-(1-3)-+ a-D-Glcp-(1-3)-+
| |
-4)-b-D-GalpNAc-(1-2)-a-D-Manp-(1-3)-a-L-Fucp-(1-3)-a-D-GalpNAc-(1- |
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Structure type: suggested polymer biological repeating unit
Compound class: O-polysaccharide, O-antigen
Reference(s) to other database(s): GTC:G41918HP, GlycomeDB:
25322
- Compound ID: 2338
|
-4)-b-D-GalpNAc-(1-2)-a-D-Manp-(1-3)-a-L-Fucp-(1-3)-a-D-GalpNAc-(1- |
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Structure type: suggested polymer biological repeating unit
Compound class: O-polysaccharide
Reference(s) to other database(s): GTC:G47233DE, GlycomeDB:
25421
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