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1. (Article ID: 7992)
 
Sassaki GL, Machado MJ, Tischer CA, Gorin PA, Iacomini M
Glycosyldiacylglycerolipids from the lichen Dictyonema glabratum
Journal of Natural Products 62(6) (1999) 715-717
 

Three glycolipids (1-3) were isolated from the basidiolichen Dictyonema glabratum. Their carbohydrate and lipid components were structurally characterized using 1D 1H and 13C and 2D NMR spectroscopy, complemented by mass spectrometry, as were the carbohydrate moieties formed on saponification. These were O-α-D-Galp-(1''→6')-O-β-D-Galp-(1'<→1)-2, 3-diacyl-D-glycerol (2) and two others not previously found in lichens, O-β-D-Galp-(1'<→1)-2,3-diacyl-D-glycerol (1) and O-α-D-Galp-(1'''→6'')-O-α-D-Galp-(1' '→6')-O-β-D-Galp-(1'<→1)-2,3-diacyl-D-glycerol (3). Each was saponified to give the free carbohydrates and its fatty acid methyl esters. The most abundant fatty acid esters in 1-3 was palmitic C16:0, but there was a wide variation of ester composition. Others present were C8:0 and C14:0 in 1, C14:0, C15:0, C17:0, C18:0, C18:1 (oleic), C18:2 (linoleic), C22:0, and C24:0 in 2, and C8:0, C14:0, C18:0, C18:1 (oleic), C18:2 (linoleic), and C18:3 (linolenic) in 3. As in ascolichens, the glycolipids appear to arise from the phycobiont.

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2. (Article ID: 10855)
 
York WS, Darvill AG, McNeil M, Albersheim P
Structure of plant cell walls. Part XVI. 3-Deoxy-D-manno-2-octulosonic acid (Kdo) is a component of rhamnogalacturonan II, a pectic polysaccharide in the primary cell walls of plants
Carbohydrate Research 138 (1985) 109-126
 

3-Deoxy-d-manno-2-octulosonic acid (KDO), a sugar previously presumed to occur only as a glycosyl residue in polysaccharides produced by Gram-negative bacteria, was found to be a component of the cell walls of higher plants. In the form of the disaccharide α-l-Rhap-(1→5)-d-KDO, KDO was released by mild hydrolysis with acid from the purified cell wall polysaccharide rhamnogalacturonan II. KDO was shown to be present in purified cell walls of several plants, including dicots, a monocot, and a gymnosperm. Improved methods for detecting and quantitating KDO residues in polysaccharides were developed during this investigation.

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