The neutral exopolysaccharide EPS180 produced from sucrose by the glucansucrase GTF180 enzyme from Lactobacillus reuteri 180 was found to be a (1→3,1→6)-α-D-glucan, with no repeating units present. Based on linkage analysis, periodate oxidation, and 1D/2D (1)H and (13)C NMR spectroscopy of the intact EPS180, as well as MS and NMR analysis of oligosaccharides obtained by partial acid hydrolysis of EPS180, a composite model, that includes all identified structural features, was formulated as follows:
a-D-Glucan, Polysaccharide structural analysis, 1H NMR spectroscopy, GTF180 Glucansucrase, Lactobacillus reuteri 180
NCBI PubMed ID: 18313038Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: j.p.kamerling@uu.nl
Institutions: Bijvoet Center, Department of Bio-Organic Chemistry, Utrecht University, Utrecht, The Netherlands
Methods: 13C NMR, 1H NMR, NMR-2D, GLC-EI-MS, partial acid hydrolysis, sugar analysis, enzymatic hydrolysis, Smith degradation, MALDI-TOF MS, NMR-1D, HPAEC-PAD, permethylation
Found