The structure and conformational behaviour of the core region of the lipopolysaccharide isolated from Sinorhizobium fredii SMH12, a wide-ranging host bacterium isolated from nodulated soybean plants growing in Vietnam, have been studied. A mixture of oligosaccharides was isolated from the lipopolysaccharide by mild hydrolysis followed by size-exclusion chromatography. The structures were determined by sugar analysis, ESI-MS/MS and NMR studies. Conformational analysis was performed by molecular dynamics calculations and compared with the NMR spectroscopic data. The results indicate that the core oligosaccharide contains a disaccharide made up of -D-glucopyranuronic acid (14)-linked to D-Kdo. In solution, this disaccharide exists as an equilibrium of three different structures.
Lipopolysaccharide, conformation, structure, core, chemistry, O-antigen, O antigen, Oligosaccharides, conformational, dynamics, Rhizobium, structure elucidation, conformation analysis, inner core, disaccharide, Sinorhizobium, PDF, disaccharides, organic, Sinorhizobium fredii
Publication DOI: 10.1002/ejoc.200800613Journal NLM ID: 9805750WWW link: http://www3.interscience.wiley.com/cgi-bin/fulltext/121470458/PDFSTARTPublisher: Wiley-VCH
Correspondence: rcarvaj@us.es
Institutions: Department of Organic Chemistry, Faculty of Chemistry, University of Seville, 41012 Sevilla, Spain, Department of Protein Science, CIB-CSIC, Ramiro de Maeztu 9, 28040 Madrid, Spain
Methods: 13C NMR, 1H NMR, GLC-MS, NMR-2D, partial acid hydrolysis, sugar analysis, ESI-MS, NMR-1D, CID-MS/MS
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