The structural determination of the O-chain repeating unit of the lipopolysaccharide from the haloalkaliphilic Halomonas pantelleriensis bacterium is described. The structure of the repeating unit was suggested on the basis of chemical analysis and NMR and MS data. The 4-O-[(S)-1-carboxyethyl]-DGlcA residue has been found for the first time in a lipopolysaccharide, being previously only found in capsular polysaccharides. A comparison of the O-chain structures of Halomonas magadiensis and H. pantelleriensis is also reported. The results show that both bacteria present lipopolysaccharides containing a high number of carboxylate groups whose salification might determine a protective buffer effect on bacterium against extreme life conditions. (© Wiley-VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2006)
Lipopolysaccharide, structure, monoclonal antibody, NMR spectroscopy, mass spectrometry, O-chain, Halomonas
Publication DOI: 10.1002/ejoc.200500868Journal NLM ID: 9805750Publisher: Wiley-VCH
Correspondence: corsaro@unina.it
Institutions: Division of Structural Biochemistry, Research Center Borstel, Leibniz-Center for Medicine and Biosciences, Parkallee 1-40, 23845 Borstel (Germany), Fax: (+49) 4537-188745
Methods: 13C NMR, 1H NMR, NMR-2D, methylation, GC-MS, mild acid hydrolysis, DOC-PAGE, MALDI-TOF MS, composition analysis, HPLC, EI-MS
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