Taxonomic group: bacteria / Firmicutes
(Phylum: Firmicutes)
The structure was elucidated in this paperNCBI PubMed ID: 35740959Publication DOI: 10.3390/biom12060834Journal NLM ID: 101596414Publisher: Basel, Switzerland: MDPI
Correspondence: A. Banerjee <abanerjee
ucm.cl>; G. Cabrera-Barjas <g.cabrera
udt.cl>
Institutions: Centro de Investigacion de Estudios Avanzados del Maule, Vicerrectoria de Investigacion y Posgrado, Universidad Catolica del Maule, Talca 3466706, Chile, Centro de Biotecnologia de los Recursos Naturales (CENBio), Facultad de Ciencias Agrarias y Forestales, Universidad Catolica del Maule, Talca 3466706, Chile, Department of Biochemical Engineering, Al-khawarizmi Collage of Engineering, University of Baghdad, Baghdad 10011, Iraq, Escuela de Ingenieria en Biotecnologia, Facultad de Ciencias Agrarias y Forestales, Universidad Catolica del Maule, Talca 3466706, Chile, UGC Center of Advanced Study, Department of Botany, The University of Burdwan, Burdwan 713104, India, Department of Biotechnology, School of Bioengineering and Biosciences, Lovely Professional University, Phagwara 144411, India, Institute of Chemistry and Natural Resources, Universidad de Talca, Talca 3460000, Chile, Unidad de Desarrollo Tecnologico (UDT), Universidad de Concepcion, Av. Cordillera 2634, Parque Industrial Coronel, Coronel 3349001, Chile
Extremophilic microorganisms often produce novel bioactive compounds to survive under harsh environmental conditions. Exopolysaccharides (EPSs), a constitutive part of bacterial biofilm, are functional biopolymers that act as a protecting sheath to the extremophilic bacteria and are of high industrial value. In this study, we elucidate a new EPS produced by thermophilic Bacillus haynesii CamB6 from a slightly acidic (pH 5.82) Campanario hot spring (56.4 °C) located in the Central Andean Mountains of Chile. Physicochemical properties of the EPS were characterized by different techniques: Scanning electron microscopy- energy dispersive X-ray spectroscopy (SEM-EDS), Atomic Force Microscopy (AFM), High-Performance Liquid Chromatography (HPLC), Gel permeation chromatography (GPC), Fourier Transform Infrared Spectroscopy (FTIR), 1D and 2D Nuclear Magnetic Resonance (NMR), and Thermogravimetric analysis (TGA). The EPS demonstrated amorphous surface roughness composed of evenly distributed macromolecular lumps. GPC and HPLC analysis showed that the EPS is a low molecular weight heteropolymer composed of mannose (66%), glucose (20%), and galactose (14%). FTIR analysis demonstrated the polysaccharide nature (-OH groups, Acetyl groups, and pyranosic ring structure) and the presence of different glycosidic linkages among sugar residues, which was further confirmed by NMR spectroscopic analyses. Moreover, D-mannose α-(1→2) and α-(1→4) linkages prevail in the CamB6 EPS structure. TGA revealed the high thermal stability (240 °C) of the polysaccharide. The functional properties of the EPS were evaluated for food industry applications, specifically as an antioxidant and for its emulsification, water-holding (WHC), oil-holding (OHC), and flocculation capacities. The results suggest that the study EPS can be a useful additive for the food-processing industry.
exopolysaccharide, Bacillus, Emulsification, Antioxidants, Food Industry, additive, flocculation, oil holding, water holding
Structure type: fragment of a bigger structure
Location inside paper: Scheme 1
Compound class: EPS
Contained glycoepitopes: IEDB_130701,IEDB_136104,IEDB_143632,IEDB_144983,IEDB_152206,IEDB_983930,SB_136,SB_196,SB_44,SB_67,SB_72
Methods: 13C NMR, 1H NMR, NMR-2D, sugar analysis, FTIR, HPLC, GPC, AFM, TGA, WHC, OHC, SEM-EDS
Comments, role: major fragment of the EPS backbone
NCBI Taxonomy refs (TaxIDs): 1925021
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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