Bellich B, Teran LC, Fazli MM, Berti F, Rizzo R, Tolker-Nielsen T, Chandan V, Cescutti P The Bep gene cluster in Burkholderia cenocepacia H111 codes for a water-insoluble exopolysaccharide essential for biofilm formation Carbohydrate Polymers (2022)
120318
NCBI PubMed ID:36436859 Publication DOI:10.1016/j.carbpol.2022.120318 Journal NLM ID:8307156 Publisher: Elsevier Correspondence: pcescuttiunits.it Institutions: Costerton Biofilm Center, Department of Immunology and Microbiology, University of Copenhagen, DK-2200 Copenhagen, Denmark, GSK, Via Fiorentina 1, 53100 Siena, Italy, Department of Life Sciences, University of Trieste, via L. Giorgieri 1, Bdg. C11, 34127 Trieste, Italy
Burkholderia cenocepacia is an opportunistic pathogen isolated from cystic fibrosis patients where it causes infections that are extremely difficult to treat with antibiotics, and sometimes have a fatal outcome. Biofilm is a virulence trait of B. cenocepacia, and is associated with infection persistence and increased tolerance to antibiotics. In biofilms exopolysaccharides have an important role, conferring mechanical stability and antibiotic tolerance. Two different exopolysaccharides were isolated from B. cenocepacia H111 biofilms: a water-soluble polysaccharide rich in rhamnose and containing an L-Man residue, and a water-insoluble polymer made of glucose, galactose and mannose. In the present work, the product encoded by B. cenocepacia H111 bepA-L gene cluster was identified as the water-insoluble exopolysaccharide, using mutant strains and NMR spectroscopy of the purified polysaccharides. It was also demonstrated that the B. cenocepacia H111 wild type strain produces the water-insoluble exopolysaccharide in pellicles, thus underlining its potential importance in in vivo infections.
Methods: 1H NMR, sugar analysis, GLC, HR-MAS NMR, pellicles production, sequence analysis of gene clusters Biosynthesis and genetic data: bepA-L Comments, role: water-insoluble polysaccharide of the bepA-L gene cluster in B. cenocepacia H111 was named Bep
Related record ID(s): 20840, 20841, 20842 NCBI Taxonomy refs (TaxIDs):1055524 Reference(s) to other database(s): GTC:G54448EJ Show glycosyltransferases
Bellich B, Teran LC, Fazli MM, Berti F, Rizzo R, Tolker-Nielsen T, Chandan V, Cescutti P The Bep gene cluster in Burkholderia cenocepacia H111 codes for a water-insoluble exopolysaccharide essential for biofilm formation Carbohydrate Polymers (2022)
120318
-2)-a-L-Rhap-(1-3)-/Variants 0/-a-L-Rhap-(1-2)-a-L-Rhap-(1-
/Variants 0/ is:
a-L-Rhap-(1-3)-
OR (exclusively)
a-L-Manp-(1-3)-
NCBI PubMed ID:36436859 Publication DOI:10.1016/j.carbpol.2022.120318 Journal NLM ID:8307156 Publisher: Elsevier Correspondence: pcescuttiunits.it Institutions: Costerton Biofilm Center, Department of Immunology and Microbiology, University of Copenhagen, DK-2200 Copenhagen, Denmark, GSK, Via Fiorentina 1, 53100 Siena, Italy, Department of Life Sciences, University of Trieste, via L. Giorgieri 1, Bdg. C11, 34127 Trieste, Italy
Burkholderia cenocepacia is an opportunistic pathogen isolated from cystic fibrosis patients where it causes infections that are extremely difficult to treat with antibiotics, and sometimes have a fatal outcome. Biofilm is a virulence trait of B. cenocepacia, and is associated with infection persistence and increased tolerance to antibiotics. In biofilms exopolysaccharides have an important role, conferring mechanical stability and antibiotic tolerance. Two different exopolysaccharides were isolated from B. cenocepacia H111 biofilms: a water-soluble polysaccharide rich in rhamnose and containing an L-Man residue, and a water-insoluble polymer made of glucose, galactose and mannose. In the present work, the product encoded by B. cenocepacia H111 bepA-L gene cluster was identified as the water-insoluble exopolysaccharide, using mutant strains and NMR spectroscopy of the purified polysaccharides. It was also demonstrated that the B. cenocepacia H111 wild type strain produces the water-insoluble exopolysaccharide in pellicles, thus underlining its potential importance in in vivo infections.
Bellich B, Teran LC, Fazli MM, Berti F, Rizzo R, Tolker-Nielsen T, Chandan V, Cescutti P The Bep gene cluster in Burkholderia cenocepacia H111 codes for a water-insoluble exopolysaccharide essential for biofilm formation Carbohydrate Polymers (2022)
120318
NCBI PubMed ID:36436859 Publication DOI:10.1016/j.carbpol.2022.120318 Journal NLM ID:8307156 Publisher: Elsevier Correspondence: pcescuttiunits.it Institutions: Costerton Biofilm Center, Department of Immunology and Microbiology, University of Copenhagen, DK-2200 Copenhagen, Denmark, GSK, Via Fiorentina 1, 53100 Siena, Italy, Department of Life Sciences, University of Trieste, via L. Giorgieri 1, Bdg. C11, 34127 Trieste, Italy
Burkholderia cenocepacia is an opportunistic pathogen isolated from cystic fibrosis patients where it causes infections that are extremely difficult to treat with antibiotics, and sometimes have a fatal outcome. Biofilm is a virulence trait of B. cenocepacia, and is associated with infection persistence and increased tolerance to antibiotics. In biofilms exopolysaccharides have an important role, conferring mechanical stability and antibiotic tolerance. Two different exopolysaccharides were isolated from B. cenocepacia H111 biofilms: a water-soluble polysaccharide rich in rhamnose and containing an L-Man residue, and a water-insoluble polymer made of glucose, galactose and mannose. In the present work, the product encoded by B. cenocepacia H111 bepA-L gene cluster was identified as the water-insoluble exopolysaccharide, using mutant strains and NMR spectroscopy of the purified polysaccharides. It was also demonstrated that the B. cenocepacia H111 wild type strain produces the water-insoluble exopolysaccharide in pellicles, thus underlining its potential importance in in vivo infections.
Related record ID(s): 8434, 20840, 20842 NCBI Taxonomy refs (TaxIDs):87882 Reference(s) to other database(s): GTC:G16935RB, GlycomeDB:25352 Show glycosyltransferases
Bellich B, Teran LC, Fazli MM, Berti F, Rizzo R, Tolker-Nielsen T, Chandan V, Cescutti P The Bep gene cluster in Burkholderia cenocepacia H111 codes for a water-insoluble exopolysaccharide essential for biofilm formation Carbohydrate Polymers (2022)
120318
NCBI PubMed ID:36436859 Publication DOI:10.1016/j.carbpol.2022.120318 Journal NLM ID:8307156 Publisher: Elsevier Correspondence: pcescuttiunits.it Institutions: Costerton Biofilm Center, Department of Immunology and Microbiology, University of Copenhagen, DK-2200 Copenhagen, Denmark, GSK, Via Fiorentina 1, 53100 Siena, Italy, Department of Life Sciences, University of Trieste, via L. Giorgieri 1, Bdg. C11, 34127 Trieste, Italy
Burkholderia cenocepacia is an opportunistic pathogen isolated from cystic fibrosis patients where it causes infections that are extremely difficult to treat with antibiotics, and sometimes have a fatal outcome. Biofilm is a virulence trait of B. cenocepacia, and is associated with infection persistence and increased tolerance to antibiotics. In biofilms exopolysaccharides have an important role, conferring mechanical stability and antibiotic tolerance. Two different exopolysaccharides were isolated from B. cenocepacia H111 biofilms: a water-soluble polysaccharide rich in rhamnose and containing an L-Man residue, and a water-insoluble polymer made of glucose, galactose and mannose. In the present work, the product encoded by B. cenocepacia H111 bepA-L gene cluster was identified as the water-insoluble exopolysaccharide, using mutant strains and NMR spectroscopy of the purified polysaccharides. It was also demonstrated that the B. cenocepacia H111 wild type strain produces the water-insoluble exopolysaccharide in pellicles, thus underlining its potential importance in in vivo infections.
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