Statistical experimental designs were applied to optimize the fermentation medium for exopolysaccharide (EPS) production. Plackett-Burman design was applied to identify the significance of seven medium variables, in which sweet potato and yeast extract were found to be the significant variables for EPS production. Central composite design was applied to evaluate the optimum condition of the selected variables. Maximum EPS production of 9.3 g/L was obtained with the predicted optimal level of sweet potato 10 %, yeast extract 0.75 %, 5.5 pH, and time 100 h. The determined (R 2) value was 0.97, indicating a good fitted model for EPS production. Results of this study showed that sweet potato can be utilized as a low-cost effective substrate for pullulan production in submerged fermentation

exopolysaccharide, response surface methodology, central composite design, sweet potato

NCBI PubMed ID: 28324414
Publication DOI: 10.1007/s13205-015-0308-3
Journal NLM ID: 101565857
Publisher: Berlin: Springer
Correspondence: tamilbiogmail.com
Institutions: Department of Biotechnology, Madha Engineering College, Chennai, India, Department of Industrial Biotechnology, Government College of Technology, Coimbatore, India, Department of Chemical Engineering, School of Bioengineering, SRM University, Chennai, India
Methods: cell growth, precipitation, centrifugation
 

• Compound ID: 12430
  

  -6)-a-D-Glcp-(1-4)-a-D-Glcp-(1-4)-a-D-Glcp-(1-

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  Structure type: polymer chemical repeating unit
  Trivial name: pullulan
  Compound class: EPS, O-polysaccharide, cell wall polysaccharide, glucan, polysaccharide
  Reference(s) to other database(s): GTC:G71532WE, CCSD:45938, CBank-STR:4859, GenDB:KY767023; GenDB:KY767024
  
   
  
  Aureobasidium pullulans MTCC 2195
  CSDB ID 48424 (all data & tools)

Pullulan is a microbial exopolysaccharide produced from Aureobasidium pullulans by submerged fermentation of a medium supplemented with carbon, nitrogen and other essential nutrients. These nutrients are expensive which increase the cost of pullulan production. The requirement of alternative cost-effective substrates for pullulan production is a prerequisite. Agro-based industries generate a large volume of solid/liquid waste and its accumulation generates a severe environmental impact. These wastes are composed of carbohydrates, proteins and other constituents, and can be used as substrates for the development of low-cost processes for the production of various microbial products. This could be a good environmental friendly waste management system. Pullulan production from agro-industrial wastes can be carried out by both submerged and solid-state fermentation by A. pullulans. Owing to its unique properties, pullulan has wide applications in many food-based industries. This review highlights pullulan production from agro-industrial wastes and potential applications of pullulan in various food industries.

pullulan, Aureobasidium pullulans, submerged fermentation, solid-state fermentation, agro-industrial waste

• Compound ID: 22175
  

  -6)-a-D-Glcp-(1-4)-a-D-Glcp-(1-4)-a-D-Glcp-(1-

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  Structure type: polymer chemical repeating unit ; 45000-600000
  Trivial name: pullulan
  Compound class: glucan, polysaccharide
  Reference(s) to other database(s): GTC:G71532WE
  
   
  
  Aureobasidium pullulans P 56, Aureobasidium pullulans MTCC 2195, Aureobasidium pullulans MTCC 1991, Aureobasidium pullulans MTCC 2670, Aureobasidium pullulans RBF 4A3, Aureobasidium pullulans ATCC 42023, Aureobasidium pullulans MTCC 6994, Aureobasidium pullulans NRRLY 6220, Aureobasidium pullulans NCIM 1049, Aureobasidium pullulans 201253, Aureobasidium pullulans CCTCCM2012259, Aureobasidium pullulans HP-2001, Aureobasidium pullulans LB83, Aureobasidium pullulans AP329
  CSDB ID 50082 (all data & tools)