Pullulan is a microbial exopolysaccharide produced by polymorphic fungus Aureobasidium pullulans. Owing to its structure, consisting of α(1→4) and α(1→6) linkages, it has unique properties and hence is suitable for a variety of commercial applications in various field including food, pharmaceuticals, and biomedical. In this chapter, biosynthesis, production through microbial fermentation, and influence of fermentation conditions like pH, temperature, aeration, agitation on microbial growth, pullulan yield, pullulan purity, and molecular weight are discussed. Later, options of using various agricultural wastes as carbon source for pullulan production are considered
morphology, exopolysaccharide, pullulan, Aureobasidium pullulans, agricultural residues
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Pullulan is a microbial exopolysaccharide produced from Aureobasidium pullulans by submerged fermentation of a medium supplemented with carbon, nitrogen and other essential nutrients. These nutrients are expensive which increase the cost of pullulan production. The requirement of alternative cost-effective substrates for pullulan production is a prerequisite. Agro-based industries generate a large volume of solid/liquid waste and its accumulation generates a severe environmental impact. These wastes are composed of carbohydrates, proteins and other constituents, and can be used as substrates for the development of low-cost processes for the production of various microbial products. This could be a good environmental friendly waste management system. Pullulan production from agro-industrial wastes can be carried out by both submerged and solid-state fermentation by A. pullulans. Owing to its unique properties, pullulan has wide applications in many food-based industries. This review highlights pullulan production from agro-industrial wastes and potential applications of pullulan in various food industries.
pullulan, Aureobasidium pullulans, submerged fermentation, solid-state fermentation, agro-industrial waste
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