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1. (Article ID: 8443)
Samanta S, Nandi AK, Sen IK, Maity P, Pattanayak M, Devi KS, Khatua S, Maiti TK, Acharya K, Islam SS
Studies on antioxidative and immunostimulating fucogalactan of the edible mushroom Macrolepiota dolichaula
Carbohydrate Research 413 (2015)
22-29
A water soluble fucogalactan (PS-II) of an average molecular weight 120000 Da was isolated from the aqueous extract of an edible mushroom Macrolepiota dolichaula. It was composed of fucose, galactose and 3-O-methyl galactose in a molar ratio of nearly 1:4:1. Structural characterization of PS-II was carried out using total hydrolysis, methylation analysis, Smith degradation, and 1D/2D NMR experiments. These results indicated that the proposed repeating unit of the PS-II had a backbone chain consisting of four (1→6)- linked α-D-Galp residues, one residue methylated at O-3, and another one substituted at O-2 by (1→2)-α-D-Galp residue, which is terminated with a α-l-Fucp moiety. The PS-II exhibited the antioxidant properties in different in vitro test systems, and also showed in vitro macrophage activation in RAW 264.7 cell line as well as splenocyte and thymocyte activation in mouse cell culture medium
NMR spectroscopy, Antioxidant, immunostimulation, fucogalactan, Macrolepiota dolichaula
NCBI PubMed ID: 26074030Publication DOI: 10.1016/j.carres.2015.05.006Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: Islam SS
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Institutions: Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore, India, Department of Biotechnology, Indian Institute of Technology (IIT) Kharagpur, Kharagpur, India, Molecular and Applied Mycology and Plant Pathology Laboratory, Department of Botany, University of Calcutta, Kolkata, India
Methods: 13C NMR, 1H NMR, methylation, periodate oxidation, GLC-MS, NMR-2D, acid hydrolysis, GLC, Smith degradation, HPLC, GPC, extraction, optical rotation measurement, acetylation, methylation analysis, reduction, CC, precipitation, macrophage activity assay, phenol-sulfuric acid assay, radical scavenging assay, derivatization, Gerwig method, evaporation, centrifugation
The publication contains the following compound(s):
- Compound ID: 21074
|
a-L-Fucp-(1-2)-a-D-Galp-(1-2)-+
|
-6)-a-D-Galp-(1-6)-a-D-Galp-(1-6)-a-D-Galp-(1-6)-a-D-Galp3Me-(1- |
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Structure type: polymer chemical repeating unit
; 120000
Compound class: polysaccharide, fucogalactan
Reference(s) to other database(s): GTC:G65715UU
- Compound ID: 21075
Structure type: monomer
Reference(s) to other database(s): GTC:G96078VE
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2. (Article ID: 10872)
Krauze-Baranowska M, Cisowski W
High-performance liquid chromatographic determination of flavone C-glycosides in some species of the Cucurbitaceae family
Journal of Chromatography A 675 (1994)
240-243
Flavonoid complexes occurring in the medicinal plants Bryonia alba, Bryonia dioica and Lagenaria siceraria were found to be flavone C-glycosides. Flavonoids of these species were compared by HPLC and separation conditions were elaborated for C-glycosides using isocratic and gradient elution. The content of the major C-glycoside, saponarin, was determined. The highest saponarin level (2.481%) was found in Bryonia dioica.
Publication DOI: 10.1016/0021-9673(94)85278-2Journal NLM ID: 9318488Publisher: Amsterdam; New York: Elsevier
Institutions: Department of Pharmacognosy, Medical Academy, Gdańsk, Poland
Methods: HPLC, UV
The publication contains the following compound(s):
- Compound ID: 27061
Structure type: monomer
Trivial name: lutonarin
Compound class: glycoside
Reference(s) to other database(s): CCSD:
48346, CBank-STR:1131
- Compound ID: 27062
Structure type: monomer
Trivial name: saponarin
Compound class: glycoside
Reference(s) to other database(s): CCSD:
48347, CBank-STR:1122
- Compound ID: 27065
Structure type: monomer
Trivial name: vitexin
Compound class: glycoside
- Compound ID: 27066
Structure type: monomer
Trivial name: isovitexin
Compound class: glycoside
- Compound ID: 27064
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b-D-1dGlc-(1C-6)-+
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Caf-(9-?)-b-D-Glcp-(1-7)-Apigenin
Caf = caffeic acid |
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Structure type: oligomer
Trivial name: saponarin caffeic ester
Compound class: glycoside
Reference(s) to other database(s): CCSD:
48349, CBank-STR:2317
- Compound ID: 27067
Structure type: monomer
Trivial name: isoorientin
Compound class: glycoside
- Compound ID: 27063
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b-D-Glcp-(1-7)-+
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b-D-1dGlcp-(1C-6)-Apigenin
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D-Glcp-(1-4')-+ |
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Structure type: oligomer
Trivial name: saponarin 4'-O-glucoside
Compound class: C-glycoside, O-glycoside
Reference(s) to other database(s): CCSD:
48348, CBank-STR:1712
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