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1. (Article ID: 8443)
Samanta S, Nandi AK, Sen IK, Maity P, Pattanayak M, Devi KS, Khatua S, Maiti TK, Acharya K, Islam SS
Studies on antioxidative and immunostimulating fucogalactan of the edible mushroom Macrolepiota dolichaula
Carbohydrate Research 413 (2015)
22-29
A water soluble fucogalactan (PS-II) of an average molecular weight 120000 Da was isolated from the aqueous extract of an edible mushroom Macrolepiota dolichaula. It was composed of fucose, galactose and 3-O-methyl galactose in a molar ratio of nearly 1:4:1. Structural characterization of PS-II was carried out using total hydrolysis, methylation analysis, Smith degradation, and 1D/2D NMR experiments. These results indicated that the proposed repeating unit of the PS-II had a backbone chain consisting of four (1→6)- linked α-D-Galp residues, one residue methylated at O-3, and another one substituted at O-2 by (1→2)-α-D-Galp residue, which is terminated with a α-l-Fucp moiety. The PS-II exhibited the antioxidant properties in different in vitro test systems, and also showed in vitro macrophage activation in RAW 264.7 cell line as well as splenocyte and thymocyte activation in mouse cell culture medium
NMR spectroscopy, Antioxidant, immunostimulation, fucogalactan, Macrolepiota dolichaula
NCBI PubMed ID: 26074030Publication DOI: 10.1016/j.carres.2015.05.006Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: Islam SS
yahoo.com>
Institutions: Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore, India, Department of Biotechnology, Indian Institute of Technology (IIT) Kharagpur, Kharagpur, India, Molecular and Applied Mycology and Plant Pathology Laboratory, Department of Botany, University of Calcutta, Kolkata, India
Methods: 13C NMR, 1H NMR, methylation, periodate oxidation, GLC-MS, NMR-2D, acid hydrolysis, GLC, Smith degradation, HPLC, GPC, extraction, optical rotation measurement, acetylation, methylation analysis, reduction, CC, precipitation, macrophage activity assay, phenol-sulfuric acid assay, radical scavenging assay, derivatization, Gerwig method, evaporation, centrifugation
The publication contains the following compound(s):
- Compound ID: 21074
|
a-L-Fucp-(1-2)-a-D-Galp-(1-2)-+
|
-6)-a-D-Galp-(1-6)-a-D-Galp-(1-6)-a-D-Galp-(1-6)-a-D-Galp3Me-(1- |
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Structure type: polymer chemical repeating unit
; 120000
Compound class: polysaccharide, fucogalactan
Reference(s) to other database(s): GTC:G65715UU
- Compound ID: 21075
Structure type: monomer
Reference(s) to other database(s): GTC:G96078VE
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2. (Article ID: 8449)
Wang D, Chen F, Wei G, Jiang M, Dong M
The mechanism of improved pullulan production by nitrogen limitation in batch culture of Aureobasidium pullulans
Carbohydrate Polymers 127 (2015)
325-331
Batch culture of Aureobasidium pullulans CCTCC M 2012259 for pullulan production at different concentrations of ammonium sulfate and yeast extract was investigated. Increased pullulan production was obtained under nitrogen-limiting conditions, as compared to that without nitrogen limitation. The mechanism of nitrogen limitation favoring to pullulan overproduction was revealed by determining the activity as well as gene expression of key enzymes, and energy supply for pullulan biosynthesis. Results indicated that nitrogen limitation increased the activities of α-phosphoglucose mutase and glucosyltransferase, up-regulated the transcriptional levels of pgm1 and fks genes, and supplied more ATP intracellularly, which were propitious to further pullulan biosynthesis. The economic analysis of batch pullulan production indicated that nitrogen limitation could reduce more than one third of the cost of raw materials when glucose was supplemented to a total concentration of 70 g/L. This study also helps to understand the mechanism of other polysaccharide overproduction by nitrogen limitation
pullulan, Aureobasidium pullulans, nitrogen limitation, enzyme activity, 46 gene expression, ATP supply
NCBI PubMed ID: 25965490Publication DOI: 10.1016/j.carbpol.2015.03.079Journal NLM ID: 8307156Publisher: Elsevier
Correspondence: dongms
njau.edu.cn
Institutions: College of Food Science and Technology, Nanjing Agricultural University, Nanjing, China, School of Biology and Basic Medical Sciences, Soochow University, Suzhou, China, State Key Laboratory of Materials-Oriented Chemical Engineering, Nanjing University of Technology, Nanjing, China
Methods: HPLC, UV, RT-PCR, cell growth, enzymatic assay, Bradford method, sonication, centrifugation
The publication contains the following compound(s):
- Compound ID: 12430
Structure type: polymer chemical repeating unit
Trivial name: pullulan
Compound class: EPS, O-polysaccharide, cell wall polysaccharide, glucan, polysaccharide
Reference(s) to other database(s): GTC:G71532WE, CCSD:
45938, CBank-STR:4859, GenDB:KY767023; GenDB:KY767024
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