The effect of Tween 80 on the fermentative production of polymalic acid (PMA) and pullulan using Aureobasidium pullulans CCTCC M2012223 was investigated. Tween 80 is beneficial for the biosynthesis of PMA and pullulan, and can regulate the ratio of PMA to pullulan in a dose-dependent manner. After adding 0.05 % Tween 80 to the media, the maximal PMA and pullulan production was 46.45 and 28.8 g/L at 60 h in a 5 L fermenter, with an increase of 75.08 and 27.21 % when compared to the control. Tween 80 could regulate and enhance oxygen uptake rate and carbon dioxide evolution rate in the early phase of fermentation, and change the cell morphology. The transcription levels of mitochondrial dicarboxylate transporter and transmembrane transporter were also dramatically upregulated. The present work will be helpful in deeply understanding the mechanism of Tween 80 on the effect of PMA and pullulan production

pullulan, Aureobasidium pullulans, gene transcription, polymalic acid, Tween 80

• Compound ID: 12430
  

  -6)-a-D-Glcp-(1-4)-a-D-Glcp-(1-4)-a-D-Glcp-(1-

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  Structure type: polymer chemical repeating unit
  Trivial name: pullulan
  Compound class: EPS, O-polysaccharide, cell wall polysaccharide, glucan, polysaccharide
  Reference(s) to other database(s): GTC:G71532WE, CCSD:45938, CBank-STR:4859, GenDB:KY767023; GenDB:KY767024
  
   
  
  Aureobasidium pullulans CCTCC M2012223
  CSDB ID 48468 (all data & tools)