In this paper, a polysaccharide fraction (FMPS) was purified from the floral mushroom cultivated in Huangshan Mountain for the first time. Physicochemical properties and antioxidant activities of FMPS were investigated. FMPS had an average molecular weight of 720000 Da and was composed of glucose. On the basis of FT-IR, NMR and methylation analysis, the repeating unit of FMPS was established as (1→3)-linked β-D-glucopyranosyl backbone with 1-linked β-D-glucopyranosyl branches substituted at O-6 position of (1→3)-linked β-D-glucopyranosyl residues. The advanced structure studies indicated that FMPS was a triple-helical polysaccharide. The main hydrodynamic radius (Rh) of FMPS was 23.4 nm and it could form a stable system with water in 0.012 g/mL solutions. In addition, FMPS exhibited high DPPH radical scavenging activities (79.46% at 5mg/mL) and hydroxyl radical scavenging activities (74.18% at 5 mg/mL), as well as Fe2+ chelating activities and ABTS radical scavenging activities to some extent
NCBI PubMed ID: 26256181Publication DOI: 10.1016/j.carbpol.2015.05.052Journal NLM ID: 8307156Publisher: Elsevier
Correspondence: junhuiwang
hfut.edu.cn
Institutions: School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei, China, Center of Analysis and Measurement, Hefei University of Technology, Hefei, China
Methods: 13C NMR, methylation, IR, GC-MS, acid hydrolysis, GC, viscosity measurement, extraction, methylation analysis, reduction, dialysis, dynamic light scattering, HPGPC, precipitation, radical scavenging assay, evaporation, Congo Red assay, Sevag method, centrifugation, ELSD
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