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Structure type: polymer chemical repeating unit
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_130648,IEDB_130701,IEDB_136045,IEDB_137473,IEDB_1391961,IEDB_141584,IEDB_142489,IEDB_144562,IEDB_144983,IEDB_152206,IEDB_152214,IEDB_174333,IEDB_885822,IEDB_983930,SB_44,SB_67,SB_72,SB_86

• Article ID: 3689
  Gajdus J, Kaczyński Z, Smietana J, Stepnowski P "Structural determination of the O-antigenic polysaccharide from Salmonella Mara (O:39)" - Carbohydrate Research 344(8) (2009) 1054-1057
  

  The O-antigenic polysaccharide of Salmonella Mara O:39 (formerly Q) was investigated by sugar and methylation analyses, absolute configuration assignment, mass spectrometry and NMR spectroscopy. The experiments revealed an O-polysaccharide chain composed of the following linear tetrasaccharide repeating units with the structure: →2)-α-L-Quip3NAc-(1→3)-α-D-Manp-(1→3)-α-L-Fucp-(1→3)-α-D-GalpNAc-(1→ where α-L-Quip3NAc is the residue of 3-acetamido-3,6-dideoxy-α-L-glucopyranose. This repeating unit is the first published structure of the O-polysaccharide from 27 serotypes of Salmonella bacteria belonging to serogroup O:39 in the Kauffmann-White classification system

  NMR, O-antigen, 3-Acetamido-3, 6-dideoxy-L-glucose, Salmonella Mara, serogroup O:39

  NCBI PubMed ID: 19394591
  Journal NLM ID: 0043535
  Publisher: Elsevier
  Correspondence: Z. Kaczynski
  Institutions: Faculty of Chemistry, University of Gdansk, Gdansk, Poland
  Methods: 13C NMR, 1H NMR, methylation, GLC-MS, sugar analysis, mild acid hydrolysis, NMR-1D
  
   
  
  Salmonella enterica ssp. enterica sv. Mara O39
  CSDB ID 23699 (all data & tools)
• Article ID: 4291
  Dziadziuszko H, Kumirska J, Muza S, Czerwicka M, Lubecka EA, Stepnowski P, Kunikowska D "Immunochemical studies of Salmonella Dakar and Salmonella Telaviv O-antigens (serogroup O:28)" - FEMS Microbiology Letters 326(1) (2012) 55-61
  

  Salmonella Dakar and Salmonella Telaviv bacteria belong to serogroup O:28, which represents 107 serovars and possesses only the epitope O28. Salmonella Telaviv has the subfactors O28(1) and O28(2) , whereas S. Dakar has O28(1) and O28(3) . So far, only limited serological and immunological information for this serogroup is available in the literature. Knowledge of the structures of their O-polysaccharides and the immunochemical investigations performed in this work allowed to reveal the nature of subfactor O28(1) as attributed to the presence of 3-linked (or 3,4-disubstituted) α-D-GalpNAc in the main chains of S. Dakar and S. Telaviv O-polysaccharides. An explanation for the cross-reactions between Salmonella enterica O28 O-antigens and other Salmonella O-polysaccharides and their structural similarity to Escherichia coli O-serogroups is also given.

  monoclonal antibody, Serogroup O:28, Salmonella bacterial strains, immunochemical study, O-antigen (OPS), epitope O28

  NCBI PubMed ID: 22092663
  Publication DOI: 10.1111/j.1574-6968.2011.02431.x
  Journal NLM ID: 7705721
  Publisher: Blackwell Publishing
  Correspondence: kumirska@ chem.univ.gda.pl
  Institutions: Department of Molecular Microbiology and Serology, National Salmonella Centre, Medical University of Gdansk, Gdansk, Poland
  Methods: SDS-PAGE, ELISA, serological methods, periodate oxidation
  
   
  
  Salmonella enterica ssp. enterica sv. Mara O39
  CSDB ID 28328 (all data & tools)

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Structure type: polymer chemical repeating unit
Contained glycoepitopes: IEDB_136105,IEDB_141807,IEDB_142488,IEDB_146664,IEDB_151531,IEDB_225177,IEDB_885823,IEDB_983931,SB_192

• Article ID: 3846
  Gajdus J, Glosnicka R, Szafranek J "Primary structure of Salmonella spp. O-antigens" - Wiadomosci Chemiczne [Polish] 60(9-10) (2006) 621-653
  

  Salmonella spp. are pathogenic Gram-negative bacteria that belong to Enterobacteriaceae family with lipopolysaccharide (LPS) as a constituent of cell wall. This is an integral component of the outer membrane of the wall. Salmonella smooth (S) forms produce LPS, which is composed of three parts, chemically bonded together viz. polysaccharide O-antigen, oligosaccharide core region and lipid A. Antigens O (O-PS) together with H flagella antigens are the foundation of serological classification of these bacteria. O-chain, which is built with up to 50 oligosaccharide repeating units, is one of the products of mild acidic hydrolysis of LPS. Due to the fact that polysaccharide antigens are the sites of specific antibody complexing, any difference in primary and secondary structures of O-antigens reflect serological specificity of bacteria. Taking this fact into consideration, we can distinguish about 2541 Salmonella serotypes with O and H antigenic formulas defined [4]. In this review we present 55 chemical structures of O-antigenic repeating units of Salmonella strains including their heterogeneity structures. The structures can have 22 different monosaccharide residues usually in 3 to 6 sugar repeating units. We describe here selected chemical and spectroscopic (MS, NMR) methods for primary structure examination of these bacterial O-PS. Enzymatic and immunochemical methods are also described. Cross-reactions of Salmonella spp. with any other bacteria or blood group A, B, 0 antigens are explained on the molecular level. Thus, structural assignments of somatic antigens of Salmonella spp. allow us to understand the molecular level of the classification system of these bacteria.

  NMR spectroscopy, O-antigens, Salmonella, MS, primary structure

  WWW link: http://baztech.icm.edu.pl/baztech/cgi-bin/btgetdoc.cgi?BUS2-0016-0014
  Publisher: Polish Chemical Society
  Correspondence: jerzyg@chemik.chem.univ.gda.pl
  Institutions: Wydzial Chemii, Uniwersytet Gdanski, ul. Sobieskiego 18, 80-952 Gdansk
  
   
  
  Salmonella dakar M (O28)
  CSDB ID 25633 (all data & tools)