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1. (Article ID: 8466)
 
Sandra K, Van Beeumen J, Stals I, Sandra P, Claeyssens M, Devreese B
Characterization of cellobiohydrolase I N-glycans and differentiation of their phosphorylated isomers by capillary electrophoresis−Q-trap mass spectrometry
Analytical Chemistry 76(19) (2004) 5878-5886
 

A capillary electrophoresis−mass spectrometric (CE−MS) method is described for the simultaneous analysis of uncharged and charged glycans. The glycans were labeled with the negatively charged tag 8-aminopyrene-1,3,6-trisulfonate by reductive amination and separated in an ammonium acetate buffer. A Q-Trap instrument was used for mass spectrometric detection. The CE−MS method was first optimized using maltooligosaccharides and ribonuclease B N-glycans and then applied to the characterization of enzymatically released N-glycans from the glycoprotein cellobiohydrolase I. The method, as developed, allowed differentiation of phosphorylated isomers and MS/MS provided useful structural information. Further structural evidence was obtained by studying the methylated glycans in off-line ESI-MS/MS experiments and by using a combination of chemical and enzymatic sequencing

glycans, cellobiohydrolase I, Q-Trap

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2. (Article ID: 9213)
 
Wang Z-R, Yang H-X, Peng X-P, Li G, Lou H-X
Induced production of zinniol analogues by co-cultivation of two endophytic fungi in the same ecological niche
Phytochemistry Letters 35 (2020) 206-210
 

Three zinniol analogues, pleoniols A-C (1-3), together with a known compound (4), were isolated from a mixed fermentation of two endophytic fungi, Pleosporales sp. F46 and Acremonium pilosum F47, both of which are found the pedicel of the medicinal plant Mahonia fortunei. High-performance liquid chromatography analysis showed that none of compounds 1-4 were detected when either one of the two fungi was cultured alone. The structures of compounds 1-4, including the absolute configurations, were deduced based on the interpretation of HRMS, NMR, and optical rotation data. Compounds 1-3 exhibited no antimicrobial efficacies against four Gram-positive or Gram-negative bacteria and two plant pathogenic fungi. Moreover, they demonstrated no obvious cytotoxic activities against four cancer cell lines, A549, MDA-MB-231, CT-26 and MCF-7. Our work indicates that the co-culture of endophytes in the same ecological niche is an effective approach for promoting interspecies interaction and enriching chemical diversity.

endophytes, bioactivity, secondary metabolites, co-cultivation, zinniol analogues

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