Huo S, Li X, Wang S, Wu P, Nan D, Rao C, Li Q, Mao X, Yan J Characterization of Burkholderia pseudomallei O antigens in different clinical strains International Journal of Biological Macromolecules225 (2023)
795-808
/Variants 0/-+
|
-3)-b-D-Glcp-(1-3)-a-L-6dTalp4(%)Ac-(1-
/Variants 0/ is:
Ac-2)-
OR (exclusively)
?%Me-2)-
NCBI PubMed ID:36402383 Publication DOI:10.1016/j.ijbiomac.2022.11.143 Journal NLM ID:7909578 Publisher: Butterworth-Heinemann Correspondence: X. Mao <maoxh2012hotmail.com>; J. Yan <zijie1011yeah.net> Institutions: Department of Clinical Microbiology and Immunology, College of Pharmacy and Medical Laboratory, Army Medical University (Third Military Medical University), Chongqing, China
O antigen is the major component of lipopolysaccharide LPS. The chemical structure of the O antigen determines the LPS serospecificity of the bacteria, and the diversity of O antigen is the basis for serotyping Burkholderia pseudomallei. In this study, structural elucidation of type B O antigen obtained from a clinical B. pseudomallei strain was conducted, and the effects of different types of LPS on macrophage differentiation were investigated. The O antigen was found to be composed of repeating units of [→4)-α-L-Rhap(1→4)-α-L-Rhap(1→2)-α-L-Rhap(1→2)-α-L-Rhap(1→3)-α-L-Rhap(1→3)-α-L-Rhap(1→4)-α-L-Rhap(1→6)-α-D-Galp(1→]n, where some of the →4)-α-L-Rhap(1 → units were substituted at O-3 by β-D-Xylp(1→ residues, and minor →3)-α-L-Rhap(1→ units were substituted at O-2 by β-D-Xylp(1→ residues. Meahwhile, the →6)-α-D-Galp(1→ units were substituted at O-3 by α-D-Galp(1→ residues. Furthermore, both type A and type B O antigens of B. pseudomallei could polarize macrophages toward the M1 phenotype, but the core oligosaccharides had no such activity. Therefore, we deduced that this polarization relies on the O antigen of LPS and might be related to the ability of B. pseudomallei to survive and replicate within macrophages. Thus, the characterization of different types of O antigen structural motifs is essential for further clarifying the persistence/survival mechanisms and inflammatory potential of B. pseudomallei.
Structure type: polymer chemical repeating unit Location inside paper: p. 804 Trivial name: type A OPS Compound class: O-polysaccharide, O-antigen Contained glycoepitopes:IEDB_142488,IEDB_146664,IEDB_983931,SB_192
Huo S, Li X, Wang S, Wu P, Nan D, Rao C, Li Q, Mao X, Yan J Characterization of Burkholderia pseudomallei O antigens in different clinical strains International Journal of Biological Macromolecules225 (2023)
795-808
The structure was elucidated in this paper NCBI PubMed ID:36402383 Publication DOI:10.1016/j.ijbiomac.2022.11.143 Journal NLM ID:7909578 Publisher: Butterworth-Heinemann Correspondence: X. Mao <maoxh2012hotmail.com>; J. Yan <zijie1011yeah.net> Institutions: Department of Clinical Microbiology and Immunology, College of Pharmacy and Medical Laboratory, Army Medical University (Third Military Medical University), Chongqing, China
O antigen is the major component of lipopolysaccharide LPS. The chemical structure of the O antigen determines the LPS serospecificity of the bacteria, and the diversity of O antigen is the basis for serotyping Burkholderia pseudomallei. In this study, structural elucidation of type B O antigen obtained from a clinical B. pseudomallei strain was conducted, and the effects of different types of LPS on macrophage differentiation were investigated. The O antigen was found to be composed of repeating units of [→4)-α-L-Rhap(1→4)-α-L-Rhap(1→2)-α-L-Rhap(1→2)-α-L-Rhap(1→3)-α-L-Rhap(1→3)-α-L-Rhap(1→4)-α-L-Rhap(1→6)-α-D-Galp(1→]n, where some of the →4)-α-L-Rhap(1 → units were substituted at O-3 by β-D-Xylp(1→ residues, and minor →3)-α-L-Rhap(1→ units were substituted at O-2 by β-D-Xylp(1→ residues. Meahwhile, the →6)-α-D-Galp(1→ units were substituted at O-3 by α-D-Galp(1→ residues. Furthermore, both type A and type B O antigens of B. pseudomallei could polarize macrophages toward the M1 phenotype, but the core oligosaccharides had no such activity. Therefore, we deduced that this polarization relies on the O antigen of LPS and might be related to the ability of B. pseudomallei to survive and replicate within macrophages. Thus, the characterization of different types of O antigen structural motifs is essential for further clarifying the persistence/survival mechanisms and inflammatory potential of B. pseudomallei.
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hotmail.com>; J. Yan <zijie1011