Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Escherichia coli [ICD11:
XN6P4 
]
NCBI PubMed ID: 34893224Publication DOI: 10.1016/j.carbpol.2021.118796Journal NLM ID: 8307156Publisher: Elsevier
Correspondence: Di Huang <huangdi
nankai.edu.cn>; Lu Feng <fenglu63
nankai.edu.cn>
Institutions: Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Nankai University, Tianjin, PR China, TEDA Institute of Biological Sciences and Biotechnology, Tianjin Key Laboratory of Microbial Functional Genomics, Nankai University, Tianjin, PR China
Extraintestinal pathogenic Escherichia coli O1 is a frequently identified serotype that causes serious infections and is often refractory to antimicrobial therapy. Glycoconjugate vaccine represents a promising measure to reduce ExPEC infections. Herein, we designed an O1-specific glyco-optimized chassis strain for manufacture of O-polysaccharide (OPS) antigen and OPS-based bioconjugate. Specifically, OPS and OPS-based glycoprotein were synthesized in glyco-optimized chassis strain, when compared to the unmeasurable level of the parent strain. The optimal expression of oligosaccharyltransferase and carrier protein further improved the titer. MS analysis elucidated the correct structure of resulting bioconjugate at routine and unreported glycosylation sequons of carrier protein, with a higher glycosylation efficiency. Finally, purified bioconjugate stimulated mouse to generate specific IgG antibodies and protected them against virulent ExPEC O1 challenge. The plug-and-play glyco-optimized platform is suitable for bioconjugate synthesis, thus providing a potential platform for future medical applications.
mass spectrometry, glycoconjugate vaccine, glyco-optimized chassis strain, glycosylation efficiency, glycosylation sequon
Structure type: polymer chemical repeating unit
Location inside paper: Fig. 1C
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_135610,IEDB_135813,IEDB_136105,IEDB_137340,IEDB_141807,IEDB_151531,IEDB_225177,IEDB_885813,IEDB_885823
Methods: PCR, SDS-PAGE, ELISA, genetic methods, LC-MS/MS, immunization, bioconjugate vaccine production
Biosynthesis and genetic data: wzy, wekM,wekN, wekN, wekO,
NCBI Taxonomy refs (TaxIDs): 562Reference(s) to other database(s): GTC:G57484QO, GlycomeDB:
6035
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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