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1. (Article ID: 8483)
 
Fukuda K, Hamada A
Purification and chemical characterization of polysaccharides obtained from Lampteromyces japonicus by concanavalin A-sepharose affinity chromatography
Biochimica et Biophysica Acta 538(3) (1978) 580-592
 

Two classes of neutral polysaccharide which could not be separated from each other by conventional methods were isolated from the fungus, Lampteromyces japonicus, by affinity chromatography using concanavalin A-Sepharose. The polysaccharide retained on the concanavalin A-Sepharose column was eluted with 0.05 M methyl α-D-mannopyranoside and appeared to be α-mannan, while that which passed through the column was virtually all β-glucan. Both polysaccharides were subjected to Smith-type degradation, methylation, acetolysis and glucosidase treatment. The results indicated that the α-mannan contained predominantly α-(1→2)-linked side chains branching from an α-(1→6)-linked backbone at the (1→2,6)-linked mannopyranosyl residues. Galactose was attached to approximately one-quarter of the non-reducing mannose terminals. The β-glucan seemed to contain mainly (1→6)-linked side chains branching from a (1→3)-linked backbone at the (1→3,6)-linked glucopyranosyl residues

structure, polysaccharide, β-glucan, α-mannan, Lampteromyces japonicus

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