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1. (Article ID: 8488)
Li N, Li L, Fang JC, Wong JH, Ng TB, Jiang Y, Wang CR, Zhang NY, Wen TY, Qu LY, Lv PY, Zhao R, Shi B, Wang YP, Wang XY, Liu F
Isolation and identification of a novel polysaccharide-peptide complex with antioxidant, anti-proliferative and hypoglycaemic activities from the abalone mushroom
Bioscience Reports 32(3) (2012)
221-228
A novel antioxidant polysaccharide-peptide complex LB-1b from the fruiting bodies of the edible abalone mushroom (Pleurotus abalonus) was purified and identified. The structural characteristic of LB-1b was identified by FTIR (Fourier-transform IR), 13C NMR and 1H NMR spectroscopy. LB-1b is a polysaccharide-peptide complex that contains glucose, rhamnose, glucuronic acid and galactose in the molar ratio of 22.4:1:1.7:1.6 and the N-terminal sequence of its peptide moiety has also been determined. The N-terminal amino acid sequence of LB-1b, IPKERKEFQQAQHLK, showed some resemblance to antioxidant enzymes. LB-1b exhibited high antioxidant activity in erythrocyte haemolysis in vitro and the anti-proliferative activity towards hepatoma HepG2 cells and breast cancer MCF7 cells with an IC50 of 24 and 14 μM respectively. LB-1b also demonstrated hypoglycaemic activity in drug-induced diabetic mice and anti-HIV-1 RT (reverse transcriptase) with an IC50 value of 12.5 μM
biological activity, Structural characterization, Pleurotus abalonus (abalone mushroom), polysaccharide-peptide complex
NCBI PubMed ID: 21810081Publication DOI: 10.1042/BSR20110012Journal NLM ID: 8102797Publisher: London: Portland Press on behalf of the Biochemical Society
Correspondence: Ng TB
mailserv.cuhk.edu.hk>; Liu F nankai.edu.cn>
Institutions: School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, China, Key Laboratory of Systems Microbial Biotechnology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China, Department of Microbiology, College of Life Science, Nankai University, Tianjin, China, Research Center of Fishery Production Standard and Quality Chinese Academy of Fishery Sciences, Beijing, China
Methods: 13C NMR, 1H NMR, IR, SDS-PAGE, inhibition studies, acid hydrolysis, amino acid analysis, biological assays, HPLC, FPLC, extraction, CC, precipitation, derivatization, antitumor activity assay, evaporation, anthrone-sulfuric acid assay, amino acid sequence analysis, Lowry method
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