Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Pseudomonas aeruginosa [ICD11:
XN5L6 
]
The structure was elucidated in this paperNCBI PubMed ID: 35618750Publication DOI: 10.1038/s42003-022-03453-2Journal NLM ID: 101719179Publisher: London, UK: Nature Publishing Group
Correspondence: P L. Howell <howell
sickkids.ca>; D.C. Sheppard <don.sheppard
mcgill.ca>
Institutions: Department of Microbiology and Immunology, Faculty of Medicine, McGill University, Montreal, QC Canada, Infectious Disease in Global Health Program, McGill University Health Centre, Montreal, QC Canada, McGill Interdisciplinary Initiative in Infection and Immunity, Montreal, QC Canada, Program in Molecular Medicine, Research Institute The Hospital for Sick Children, Toronto, ON Canada, Department of Biochemistry, University of Toronto, Toronto, ON Canada, Department of Microbiology, University of Washington, Seattle, WA USA, Department of Chemistry, Washington University, St. Louis, MO 63130 USA
The genetic capacity to synthesize the biofilm matrix exopolysaccharide Pel is widespread among Gram-negative and Gram-positive bacteria. However, its exact chemical structure has been challenging to determine. Using a Pseudomonas aeruginosa strain engineered to overproduce Pel, improvements to the isolation procedure, and selective hydrolysis with the glycoside hydrolase PelAh, we demonstrate that Pel is a partially de-N-acetylated linear polymer of α-1,4-N-acetylgalactosamine comprised predominantly of dimeric repeats of galactosamine and N-acetylgalactosamine.
structure, Pseudomonas aeruginosa, bacteria, hydrolysis, exopolysaccharide, Biofilm, Pel polysaccharide
Structure type: fragment of a bigger structure
Location inside paper: Fig. 1, p. 502-3
Trivial name: Pel
Compound class: EPS
Contained glycoepitopes: IEDB_130648,IEDB_137473,IEDB_1391961,IEDB_141584,IEDB_885822
Methods: 1H NMR, NMR-2D, partial acid hydrolysis, GC-MS, MALDI-TOF MS, de-N-acetylation, composition analysis, N-acetylation, enzymatic MS fingerprinting
Comments, role: Pel is predominantly constituted of a dimer repeat
NCBI Taxonomy refs (TaxIDs): 208964
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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