Taxonomic group: bacteria / Firmicutes
(Phylum: Firmicutes)
The structure was elucidated in this paperNCBI PubMed ID: 35594939Publication DOI: 10.1016/j.ijbiomac.2022.05.105Journal NLM ID: 7909578Publisher: Butterworth-Heinemann
Correspondence: mitorino
cerela.org.ar
Institutions: Centro de Referencia para Lactobacilos (CERELA)-CCT CONICET NOA Sur, Batalla de Chacabuco 145, San Miguel de Tucumán 4000, Tucumán, Argentina, Instituto de Química del Noroeste Argentino (INQUINOA) - Universidad Nacional de Tucumán (UNT) - CCT CONICET NOA Sur, Instituto de Química Física, Facultad de Bioquímica, Química y Farmacia, UNT, Batalla de Ayacucho 471, San Miguel de Tucumán 4000, Tucumán, Argentina, BIOFORGE (Group for Advanced Materials and Nanobiotechnology), University of Valladolid, CIBER-BBN, Valladolid, Spain
Steel corrosion is a global issue that affects safety and the economy. Currently, the homopolysaccharide (HoPS) structure of a novel lactic acid bacterium (LAB) is under study, as well as its application as a green corrosion inhibitor. Weissella cibaria FMy 2-21-1 is a LAB strain capable of producing HoPS in sucrose enriched media. The isolated and purified HoPS was characterized by different spectroscopic analyses as a linear α-1,6 dextran adopting a random coil conformation, with high molecular weight and extended size in water. The polysaccharide showed a semi-crystalline organization, which is a requirement for film formation. Its biocoating showed a grainy network structure, with a slightly lesser hydrophobic role in the aqueous environment than in the ionic one. The electrochemical measurements of the steel-HoPS coating showed that the biopolymer layer acts as an anodic-type corrosion inhibitor, with high resistance to corrosion by water and with chloride ions which prevent pitting, a corrosion process typical of bare steel. Few reports have cited the application of LAB HoPS as corrosive coating inhibitors. This work is the first to explore the influence of a structurally characterized dextran from Weissella cibaria strain as a potential steel corrosion inhibitor in ionic environments.
Lactic acid bacteria, LAB, biocoating, corrosion inhibitor, HoPS structure, Weissella cibaria
Structure type: homopolymer
Location inside paper: p. 196, Fig. 1C
Trivial name: glucan, α-D-glucan, dextran, α-1,6-glucan, α-(1,6)-glucan, dextran, α-(1,6)-glucan, α-1,6 dextran, β-1,3-D-glucan, polysaccharide IOP
Compound class: EPS, O-polysaccharide, cell wall polysaccharide, glucan, polysaccharide
Contained glycoepitopes: IEDB_142488,IEDB_144998,IEDB_146664,IEDB_158538,IEDB_983931,SB_192
Methods: 13C NMR, 1H NMR, NMR-2D, UV, CD, statistical analysis, Congo Red assay, AFM, XRD, DLS, SLS, FT-R, SERS, electrochemical measurements
NCBI Taxonomy refs (TaxIDs): 137591Reference(s) to other database(s): GTC:G69605LY, GlycomeDB:
2461
Show glycosyltransferases
NMR conditions: in DMSO-d6 at 298 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
aDGlcp 97.8 71.5 73.5 70.3 69.7 65.60
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
aDGlcp 4.83 ? ? ? ? ?
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
aDGlcp 97.8/4.83 71.5/? 73.5/? 70.3/? 69.7/? 65.60/?
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| | aDGlcp | 4.83 | ? | ? | ? | ? | ? |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| | aDGlcp | 97.8 | 71.5 | 73.5 | 70.3 | 69.7 | 65.60 |
|
There is only one chemically distinct structure:
Found 
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