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1. (Article ID: 8507)
Ye L, Li J, Zhang J, Pan Y
NMR characterization for polysaccharide moiety of a glycopeptide
Fitoterapia 81(2) (2010)
93-96
Ganoderma lucidum is a traditional medicinal herb used to treat various diseases in China and Southeast Asia for thousands of years. An aqueous glycopeptide, LZ-B-1, was prepared by successive chromatography and exhibited an immunostimulating potential. To better understand the mechanism of bioactivity for this compound, the polysaccharide moiety of glycopeptide LZ-B-1 was studied by NMR spectroscopy. The results indicated that the polysaccharide moiety had a backbone of 1,6-disubstituted-α-galactopyranosyl, 1,2,6-trisubstituted-α-galactopyranosyl, 1,3-disubstituted-β-glucopyranosyl and 1,4,6-trisubstituted-β-glucopyranosyl residues. The branches were mainly composed of 1-substituted-β-glucopyranosyl and 1-substituted-α-fucopyranosyl residues
NMR, polysaccharide, structural analysis, Ganoderma lucidum
NCBI PubMed ID: 19686815Publication DOI: 10.1016/j.fitote.2009.08.007Journal NLM ID: 16930290RPublisher: Elsevier
Correspondence: Zhang J
163.com>; Pan Y shou.edu.cn>
Institutions: Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai, China, College of Food Science, Shanghai Ocean University, Shanghai, China, Department of Biology, College of Food Science and Biotechnology, Zhejiang Gongshang University, Food Safety Key Lab of Zhejiang Province, Hangzhou, China
Methods: 13C NMR, 1H NMR, NMR-2D, HPLC, extraction, CC, phenol-sulfuric acid assay
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