Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Haemophilus influenzae [ICD11:
XN1P6 
]
NCBI PubMed ID: 36031235Publication DOI: 10.1016/S1875-5364(22)60184-5Journal NLM ID: 101504416Publisher: Beijing: Science Press; Elsevier
Correspondence: J.K. Liu <liujiankai
biominhai.com>; J. Yin <jianyin
jiangnan.edu.cn>
Institutions: Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, China, National & Local Joint Engineering Laboratory for Novel Vaccine Development Technology, Beijing Minhai Biotechnology Co., Ltd., Beijing, China, Jiangnan University-Beijing Minhai Biotechnology Joint Research Center of Carbohydrate-Based Vaccines, Jiangnan University, Wuxi, China
The ribose and phosphorus contents in Haemophilus influenzae type b (Hib) capsular polysaccharide (CPS) are two important chemical indexes for the development and quality control of Hib conjugate vaccine. A quantitative 1H- and 31P-NMR method using a single internal standard was developed for simultaneous determination of ribose and phosphorus contents in Hib CPS. Hexamethylphosphoramide (HMPA) was successfully utilized as an internal standard in quantitative 1H-NMR method for ribose content determination. The ribose and phosphorus contents were found to be affected by the concentration of polysaccharide solution. Thus, 15-20 mg·L-1 was the optimal concentration range of Hib CPS in D2O solution for determination of ribose and phosphorus contents by this method. The ribose and phosphorus contents obtained by the quantitative NMR were consistent with those obtained by traditional chemical methods. In conclusion, this quantitative 1H- and 31P-NMR method using a single internal standard shows good specificity, accuracy and precision, providing a valuable approach for the quality control of Hib glycoconjugate vaccines.
capsular polysaccharide, Haemophilus influenzae type b, phosphorus content, quantitative (1)H-NMR, quantitative (31)P-NMR, ribose content
Structure type: polymer chemical repeating unit ; 200000
Location inside paper: p. 634
Compound class: CPS
Contained glycoepitopes: IEDB_114703,IEDB_146102,IEDB_146103,IEDB_149136,IEDB_591403,IEDB_910421
Methods: guantitative NMR method, quantitative 1H NMR, quantitative 31P NMR
NCBI Taxonomy refs (TaxIDs): 727
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There is only one chemically distinct structure:
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