CSDB: Search results

Taxonomic group: bacteria / Firmicutes (Phylum: Firmicutes)

The structure was elucidated in this paper
NCBI PubMed ID: 35550745
Publication DOI: 10.1016/j.carbpol.2022.119515
Journal NLM ID: 8307156
Publisher: Elsevier
Correspondence: S.C.J. Loo <joachimloo

ntu.edu.sg>; <scloo

hsph.harvard.edu>
Institutions: School of Materials Science and Engineering, Nanyang Technological University, 50 Nanyang Avenue, 639798, Singapore, School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, 637551, Singapore, Singapore Centre for Environmental Life Sciences Engineering (SCELSE), Nanyang Technological University, 60 Nanyang Drive, 637551, Singapore, School of Biological, Earth and Environmental Sciences, The University of New South Wales, Sydney, NSW 2052, Australia

Exopolysaccharides from water kefir grains are a potential source of novel, food-safe and functional materials. Herein, prebiotic properties of polysaccharides produced by water kefir-derived Liquorilactobacillus satsumensis bacteria were explored. Strains were cultured in sucrose-supplemented media for exopolysaccharides production, and partial hydrolysis was performed to yield shorter chain polysaccharides. Structural characterization revealed that hydrolyzed polysaccharides were branched glucans comprising α-1,6 bonds and α-1,3/α-1,4 branching, with molecular weight of ~10 kDa. Hydrolyzed polysaccharides demonstrated selective utilization by probiotics, but not by pathogens, and were non-digestible by human digestive enzymes. Particularly, hydrolyzed polysaccharides were fermentable by kefir-derived probiotics, and these were combined in a novel kefir synbiotic formulation. Using large bowel simulated conditions, it was demonstrated that hydrolyzed polysaccharides and kefir synbiotics promoted Bacteroidetes abundance, and increased acetate, propionate, and butyrate concentrations. Overall, hydrolyzed glucans from Liquorilactobacillus satsumensis have prebiotic properties with enhanced benefits in a synbiotic when combined with kefir probiotics.

hydrolysis, exopolysaccharide, Dextran, Lactobacilli, microbiome, bacteria-derived, branching, synergistic

Structure type: structural motif or average structure ; 960000
Location inside paper: Fig. 1E
Trivial name: α-glucan
Compound class: EPS
Contained glycoepitopes: IEDB_140629,IEDB_142488,IEDB_144998,IEDB_146664,IEDB_158538,IEDB_2346541,IEDB_983931,SB_192

Methods: 1H NMR, PCR, partial acid hydrolysis, GC-MS, composition analysis, GPC, extraction, fermentation, sequencing, TGA, DSC, cultivation, HPLC-RID

NCBI Taxonomy refs (TaxIDs): 259059
Show glycosyltransferases

There are 64 chemically distinct structures. Please, select:

*OC[C@H]1O[C@H](OC[C@H]2O[C@H](OC[C@H]3O[C@H](OC[C@H]4O[C@H](*)[C@H](O)[C@@H](O)[C@@H]4O)[C@H](O)[C@@H](O[C@H]4O[C@H](CO)[C@@H](O)[C@H](O)[C@H]4O)[C@@H]3O)[C@H](O)[C@@H](O)[C@@H]2O[C@H]2O[C@H](CO)[C@@H](O)[C@H](O)[C@H]2O)[C@H](O)[C@@H](O)[C@@H]1O

SVG MW SMILES 3D mol Ionize