Taxonomic group: bacteria / Firmicutes
(Phylum: Firmicutes)
Associated disease: infection due to Bacillus [ICD11:
XN9ZE 
]
The structure was elucidated in this paperNCBI PubMed ID: 35287863Publication DOI: 10.1016/j.carbpol.2022.119241Journal NLM ID: 8307156Publisher: Elsevier
Correspondence: S.G. Dastager <sg.dastager
ncl.res.in>
Institutions: Academy of Scientific and Innovative Research (AcSIR), New Delhi, India, NCIM Resource Center, CSIR-National Chemical Laboratory, Pune 411008, India, Division of Organic Chemistry, CSIR-National Chemical Laboratory, Pune 411008, India, Beckman Coulter Life Sciences, Mumbai, India
In the present study, a cost-effective, robust Microbioreactor based production optimization of levan like exopolysaccharide from marine Bacillus sp. SGD-03 was analysed. FE-SEM analysis has showed the significant fibrillar structure of EPS. Size exclusion chromatography and other analytical data revealed that, produced EPS has a molecular weight of 1.0 × 104 Da and is composed of fructose monosaccharide with hydroxyl, carbonyl, and ether groups. The backbone structure of EPS has a branching pattern of β-(2,6) linkages which confirms the similarity with available levan like polymers. The cost-effective media composition for levan production was demonstrated. The maximum yield of crude levan obtained was 123.9 g/L by response surface methodology using robust BioLector Pro Microbioreactor, and same has been validated with shake flask, 1 L and 10 L pilot-scale fermentation.
structure, EPS, marine, optimization, microbioreactor
Structure type: fragment of a bigger structure ; 10000
Location inside paper: p. 119241-9, Fig. 8A, Fig. 8B
Trivial name: a levan type
Compound class: EPS
Contained glycoepitopes: IEDB_923066
Methods: 13C NMR, 1H NMR, NMR-2D, PCR, sugar analysis, TLC, FTIR, SEC-HPLC, SEM, DEPT, genome sequencing, UHPLC, FE-SEM, EPS production, data analysis, screening of EPS producing strain, LC-HRMS
Comments, role: NMR temperature was not specified
NCBI Taxonomy refs (TaxIDs): 1402856
Show glycosyltransferases
NMR conditions: in D2O
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
bDFruf 59.9 104.2 76.3 75.2 80.3 63.4
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
bDFruf 3.60-3.71 - 4.12 4.03 3.89 3.48-3.83
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
bDFruf 59.9/3.60-3.71 76.3/4.12 75.2/4.03 80.3/3.89 63.4/3.48-3.83
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| | bDFruf | 3.60
3.71 |
| 4.12 | 4.03 | 3.89 | 3.48
3.83 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| | bDFruf | 59.9 | 104.2 | 76.3 | 75.2 | 80.3 | 63.4 |
|
There is only one chemically distinct structure:
Found 
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