Taxonomic group: bacteria / Firmicutes
(Phylum: Firmicutes)
The structure was elucidated in this paperNCBI PubMed ID: 36088010Publication DOI: 10.1016/j.carbpol.2022.119971Journal NLM ID: 8307156Publisher: Elsevier
Correspondence: W. Li <lw1981
njau.edu.cn>
Institutions: College of Food Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China
Exopolysaccharide (EPS) isolated from Lactobacillus helveticus SNA12 were purified, and one fraction (SNA12-EPS) was obtained. The structure of SNA12-EPS was proposed using Fourier-transform infrared spectroscopy, gas chromatography-mass spectrometry, and nuclear magnetic resonance. Results showed that SNA12-EPS was rich in galactose and glucose with the molar ratios of 2.1:1.0, and SNA12-EPS possessed the repeat units of →3)-β-D-Glcp-(1→3)-β-D-Glcp-(1→4)-β-D-Galp-(1→ with an average molecular weight of 3.81 × 105 Da. The scanning electron microscope results showed that SNA12-EPS had a tight structure with a smooth and uneven surface. Furthermore, the prebiotic potential of SNA12-EPS was performed using in vitro simulated digestion with human faecal fermentation. SNA12-EPS was not digested by digestive juice, and it could markedly regulate the gut microbiota composition by increasing the relative abundances of Parabacteroides and Blautia and decreasing the abundance of pathogenic bacteria of Fusobacterium. Additionally, SNA12-EPS improved the ability of gut microbiota to produce short-chain fatty acids.
structure, exopolysaccharide (EPS), gut microbiota, in vitro fermentation, Lactobacillus helveticus SNA12
Structure type: polymer chemical repeating unit ; 381000
Location inside paper: abstract, Fig. 3(e) table 2
Trivial name: SNA12-EPS
Compound class: EPS
Contained glycoepitopes: IEDB_136044,IEDB_137472,IEDB_141794,IEDB_142488,IEDB_146664,IEDB_153543,IEDB_190606,IEDB_983931,SB_165,SB_166,SB_187,SB_192,SB_195,SB_7,SB_88
Methods: 13C NMR, 1H NMR, methylation, NMR-2D, GC-MS, sugar analysis, FTIR, HPLC, UV, statistical analysis, fermentation, SEM, in vitro simulated digestion of SNA12-EPS, analysis of gut microbiota
Comments, role: NMR temperature was not specified
NCBI Taxonomy refs (TaxIDs): 1587
Show glycosyltransferases
NMR conditions: in D2O
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
4,3 bDGlcp 102.48 73.11 79.20 69.20 73.56 61.28
4 bDGlcp 102.48 73.11 79.20 69.20 73.56 61.28
bDGalp 103.67 70.65 72.74 78.57 72.19 61.07
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
4,3 bDGlcp 4.64 3.70 3.78 4.06 3.95 3.78
4 bDGlcp 4.64 3.70 3.78 4.06 3.95 3.78
bDGalp 4.50 3.58 3.69 3.71 3.80 3.95
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
4,3 bDGlcp 102.48/4.64 73.11/3.70 79.20/3.78 69.20/4.06 73.56/3.95 61.28/3.78
4 bDGlcp 102.48/4.64 73.11/3.70 79.20/3.78 69.20/4.06 73.56/3.95 61.28/3.78
bDGalp 103.67/4.50 70.65/3.58 72.74/3.69 78.57/3.71 72.19/3.80 61.07/3.95
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 4,3 | bDGlcp | 4.64 | 3.70 | 3.78 | 4.06 | 3.95 | 3.78 |
| 4 | bDGlcp | 4.64 | 3.70 | 3.78 | 4.06 | 3.95 | 3.78 |
| | bDGalp | 4.50 | 3.58 | 3.69 | 3.71 | 3.80 | 3.95 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 4,3 | bDGlcp | 102.48 | 73.11 | 79.20 | 69.20 | 73.56 | 61.28 |
| 4 | bDGlcp | 102.48 | 73.11 | 79.20 | 69.20 | 73.56 | 61.28 |
| | bDGalp | 103.67 | 70.65 | 72.74 | 78.57 | 72.19 | 61.07 |
|
There is only one chemically distinct structure:
Found 
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