Taxonomic group: bacteria / Firmicutes
(Phylum: Firmicutes)
The structure was elucidated in this paperNCBI PubMed ID: 35415646Publication DOI: 10.1016/j.fochms.2021.100054Journal NLM ID: 7702639Publisher: Elsevier Applied Science Publishers
Correspondence: Wei Li <lw1981
njau.edu.cn>
Institutions: College of Food Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China
Exopolysaccharide (EPS) was produced by Lacticaseibacillus paracasei ZY-1 isolated from Tibetan kefir grains, and the preliminary structure of two EPS fractions (EPS1 and EPS2) was investigated. NMR analysis revealed that the backbone of higher producing EPS1 was consisted of →6)-α-D-Manp-(1→, →2,6)-α-D-Glcp-(1→, α-D-Manp-(1→, →2)-α-D-Glcp-(1→, →3)-α-D-Manp-(1→, →6)-α-D-Glcp-(1→. Furthermore, an eps gene cluster that encodes the glycosyltransferase and relevant proteins for EPS biosynthesis was identified on the basis of bioinformation analysis of the complete genome. RT-qPCR results indicated that wzd (ZY-1_2260) and wze (ZY-1_2259) might be essential genes involved in EPS production. Meanwhile, the synthetic mechanism of EPS1 in L. paracasei ZY-1 was further proposed. Besides, the crude and purified EPS showed certain scavenging activities against DPPH, hydroxyl and ABTS radicals. Results provided a better understanding of EPS biosynthesis in L. paracasei ZY-1 at the gene level.
biosynthesis, eps gene cluster, Structural characterization, exopolysaccharide (EPS), Lacticaseibacillus paracasei ZY-1
Structure type: polymer chemical repeating unit
Location inside paper: Fig. 3G, table 1
Compound class: EPS
Contained glycoepitopes: IEDB_130701,IEDB_141836,IEDB_142488,IEDB_144983,IEDB_144998,IEDB_146664,IEDB_152206,IEDB_164174,IEDB_232584,IEDB_983930,IEDB_983931,SB_192,SB_197,SB_44,SB_67,SB_72
Methods: 13C NMR, 1H NMR, NMR-2D, PCR, FTIR, composition analysis, HPLC, statistical analysis, RNA extraction, radical scavenging assay, Congo Red assay, genome sequencing, genome annotation, UV-vis, TA
NCBI Taxonomy refs (TaxIDs): 1597
Show glycosyltransferases
NMR conditions: in D2O at 313 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
6,2,6 aDManp 99.81 71.67 73.41 74.63 71.33 66.30
6,2,2,3 aDManp 102.22 70.11 71.33 69.85 70.73 60.71
6,2,2 aDManp 102.10 69.60 78.05 71.63 71.38 60.71
6,2 aDGlcp 100.61 78.48 70.62 73.41 71.33 67.06
6 aDGlcp 98.35 78.74 71.67 70.03 73.41 62.74
aDGlcp 98.65 71.63 73.28 70.85 73.41 67.82
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
6,2,6 aDManp 5.42 3.63 3.97 3.65 3.87 3.81
6,2,2,3 aDManp 5.17 4.10 3.87 4.26 3.90 3.85
6,2,2 aDManp 5.07 4.24 3.97 3.67 3.85 3.88
6,2 aDGlcp 5.31 4.14 3.92 3.79 3.87 3.67
6 aDGlcp 5.11 4.05 3.63 3.70 3.97 3.72
aDGlcp 4.99 3.62 3.81 3.89 3.79 3.94
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
6,2,6 aDManp 99.81/5.42 71.67/3.63 73.41/3.97 74.63/3.65 71.33/3.87 66.30/3.81
6,2,2,3 aDManp 102.22/5.17 70.11/4.10 71.33/3.87 69.85/4.26 70.73/3.90 60.71/3.85
6,2,2 aDManp 102.10/5.07 69.60/4.24 78.05/3.97 71.63/3.67 71.38/3.85 60.71/3.88
6,2 aDGlcp 100.61/5.31 78.48/4.14 70.62/3.92 73.41/3.79 71.33/3.87 67.06/3.67
6 aDGlcp 98.35/5.11 78.74/4.05 71.67/3.63 70.03/3.70 73.41/3.97 62.74/3.72
aDGlcp 98.65/4.99 71.63/3.62 73.28/3.81 70.85/3.89 73.41/3.79 67.82/3.94
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 6,2,6 | aDManp | 5.42 | 3.63 | 3.97 | 3.65 | 3.87 | 3.81 |
| 6,2,2,3 | aDManp | 5.17 | 4.10 | 3.87 | 4.26 | 3.90 | 3.85 |
| 6,2,2 | aDManp | 5.07 | 4.24 | 3.97 | 3.67 | 3.85 | 3.88 |
| 6,2 | aDGlcp | 5.31 | 4.14 | 3.92 | 3.79 | 3.87 | 3.67 |
| 6 | aDGlcp | 5.11 | 4.05 | 3.63 | 3.70 | 3.97 | 3.72 |
| | aDGlcp | 4.99 | 3.62 | 3.81 | 3.89 | 3.79 | 3.94 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 6,2,6 | aDManp | 99.81 | 71.67 | 73.41 | 74.63 | 71.33 | 66.30 |
| 6,2,2,3 | aDManp | 102.22 | 70.11 | 71.33 | 69.85 | 70.73 | 60.71 |
| 6,2,2 | aDManp | 102.10 | 69.60 | 78.05 | 71.63 | 71.38 | 60.71 |
| 6,2 | aDGlcp | 100.61 | 78.48 | 70.62 | 73.41 | 71.33 | 67.06 |
| 6 | aDGlcp | 98.35 | 78.74 | 71.67 | 70.03 | 73.41 | 62.74 |
| | aDGlcp | 98.65 | 71.63 | 73.28 | 70.85 | 73.41 | 67.82 |
|
There is only one chemically distinct structure:
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