O polysaccharides (OPS) of the lipopolysaccharides (LPS) of Pseudomonas syringae pathovar tomato GSPB 483 and pathovar maculicola IMV 381 were studied by 1H NMR and 13C NMR spectroscopy, including two-dimensional COSY, rotating-frame NOE spectroscopy (ROESY), and H-detected 1H,13C heteronuclear multiple-quantum coherence (HMQC) experiments. The OPS from both strains were shown to be chemically identical. Two structurally different types of repeating units (O repeats 1 and 2) were elucidated in each OPS. The minor O repeat is a pentasaccharide having the structure 2. The same structure has been reported earlier for some other OPS of P. syringae. The major O repeat is a hexasaccharide with the novel structure 1 which is distinguished from 2 by the presence of the second lateral residue of 3-acetamido-3,6-dideoxy-D-galactose (D-Fuc3NAc) and by a different position of substitution of one of the L-rhamnose (Rha) residues in the backbone. [structures: see text] Structural and serological diversity of OPS of strains belonging to P. syringae pv. tomato and pv. maculicola and phylogenetic relatedness of the strains are discussed.
Lipopolysaccharide, lipopolysaccharides, LPS, structure, strain, structural, characterization, polysaccharide, O-antigen, Pseudomonas, antibody, monoclonal, monoclonal antibody, O-polysaccharide, O polysaccharide, bacteria, O-specific, O-specific polysaccharide, 6-dideoxy-D-galactose, serogroup, backbone, immunochemical, 3-Acetamido-3, pathovar, Pseudomonas syringae, L-rhamnose, classification, relationship, heterogeneity
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