This study deals with the chemical characterization of a capsular polysaccharide (CPS) produced by a thermal biomass largely comprising the cyanobacterium Mastigocladus laminosus. The sugar moiety of this polymer is composed of seven neutral monosaccharides (Rha, Fuc, Ara, Xyl, Man, Glc, Gal) and two uronic acids (GalA, GlcA). Proteins represent 18% of the dry weight of the CPS. Organic acid substituents (acetate, pyruvate, succinate) were also detected and estimated by high-performance liquid chromatography. The presence of sulfate groups (5% w/w) was observed, which represents a relatively rare feature for cyanobacteria. Acidic hydrolysis of the purified polysaccharide led to the isolation of four oligosaccharidic fractions. NMR spectroscopy studies of two of the four purified oligosaccharides allowed them to be identified as: α-GlcA-(1→2)-α-GalA-(1→2)-Man and α-GlcA-(1→2)-α-GalA-(1→2)-β-Man-(1→4)-β-Gal-(1→2)-Rha?
Oligosaccharides, capsular polysaccharide, cyanobacteria
NCBI PubMed ID: 8789345Journal NLM ID: 7909578Publisher: Butterworth-Heinemann
Institutions: Laboratoire de Biologie Cellulaire Vegetale et Valorisation des Especes Ligneuses, Université de Limoges, Limoges, France
Methods: methylation, NMR-2D, NMR, sugar analysis
Found