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1. (Article ID: 8780)
Nobre C, Alves Filho EG, Fernandes FAN, Brito ES, Rodrigues S, Teixeira JA, Rodrigues LR
Production of fructo-oligosaccharides by Aspergillus ibericus and their chemical characterization
Lebensmittel-Wissenschaft und Technologie = Food Science and Technology 89 (2018)
58-64
A great demand for prebiotics is driving the search for new sources of fructo-oligosaccharides (FOS) producers and for FOS with differentiated functionalities. In the present work, FOS production by a new isolated strain of Aspergillus ibericus was evaluated. The temperature of fermentation and initial pH were optimized in shaken flask to yield a maximal FOS production, through a central composite experimental design. FOS were produced in a one-step bioprocess using the whole cells of the microorganism. The model (R2 = 0.918) predicted a yield of 0.56, experimentally 0.53 ± 0.03 gFOS.ginitial sucrose −1 was obtained (37.0 °C and a pH of 6.2). A yield of 0.64 ± 0.02 gFOS.ginitial sucrose−1 was obtained in the bioreactor, at 38 h, with a content of 118 ± 4 g.L−1 in FOS and a purity of 56 ± 3%. The chemical structure of the FOS produced by A. ibericus was determined by HPLC and NMR. FOS were identified as 1-kestose, nystose, and 1F-fructofuranosylnystose. In conclusion, A. ibericus was found to be a good alternative FOS producer.
chemical structure, fructo-oligosaccharides, experimental design, Aspergillus ibericus, production yield optimization
Publication DOI: 10.1016/j.lwt.2017.10.015Journal NLM ID: 9876251Publisher: London, etc.: Academic Press, etc.
Correspondence: Nobre C
gmail.com>; Nobre C deb.uminho.pt>; Alves Filho EG yahoo.com.br>; Fernandes FAN ufc.br>; Brito ES embrapa.br>; Rodrigues S ufc.br>; Teixeira JA deb.uminho.pt>; Rodrigues LR deb.uminho.pt>
Institutions: Centre of Biological Engineering, University of Minho, Campus de Gualtar, Braga, Portugal, LABIOTEC, Food Technology Department, Federal University of Ceará, Fortaleza, Brazil, Embrapa Agroindustria Tropical, Fortaleza, Brazil, Chemical Engineering Department, Federal University of Ceará, Campus do Pici, Fortaleza, Brazil
Methods: 13C NMR, 1H NMR, HPLC, statistical analysis, HMBC, COSY, HSQC, HPLC-ELSD
The publication contains the following compound(s):
- Compound ID: 347
Structure type: oligomer
Trivial name: 1-kestose, inulin
Compound class: fructan, fructo-oligosaccharide
Reference(s) to other database(s): GTC:G11260CN, GlycomeDB:
396, CCSD:
26122, CBank-STR:5624, GenDB:MH445969, US-PT:US2017298332A1
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2. (Article ID: 8782)
Nobre C, Sousa SC, Silva SP, Pinheiro AC, Coelho E, Vicente AA, Gomes AMP, Coimbra MA, Teixeira JA, Rodrigues LR
In vitro digestibility and fermentability of fructo-oligosaccharides produced by Aspergillus ibericus
Journal of Functional Foods 46 (2018)
278-287
The bifidogenic potential of fructo-oligosaccharides (FOS) produced by a newly isolated strain Aspergillus ibericus was studied. Their activity was compared to FOS produced by Aureobasidium pullulans and to a non microbial commercial FOS sample (Raftilose(center dot) P95). FOS fermentability by a number of probiotic bacteria and their hydrolytic resistance to the simulated harsh conditions of the digestive system was evaluated. Aspergillus ibericus FOS sample effectively promoted probiotic bacteria growth. Overall, microbial-derived FOS promoted greater cellular growth compared to the commercial sample. FOS fermentation was both substrate and strain specific. The FOS structural differences identified may explain their distinct assimilation by the probiotics. [Fru(2→6)Glc] (possibly blastose) and a reducing trisaccharide (possibly [Fru(β2→6)Glc(α1<->β2)Fru], neokestose) were only found in microbial-derived FOS samples, while Raftilosee(center dot) P95 was richer in inulobiose/inulotriose. 1-Kestose and nystose were only slightly hydrolyzed in the presence of gastric and intestinal fluid. FOS synthesized by Aspergillus exhibited great potential as food ingredients with likely prebiotic features.
fructo-oligosaccharides, probiotic, Prebiotics, Aspergillus, glycosidic linkage analysis, neoFOS
Publication DOI: 10.1016/j.jff.2018.05.004Journal NLM ID: 101513493Publisher: Amsterdam: Elsevier
Correspondence: Nobre C
gmail.com>; Nobre C deb.uminho.pt>
Institutions: Centre of Biological Engineering, University of Minho, Campus de Gualtar, Braga, Portugal, Universidade Católica Portuguesa, CBQF – Centro de Biotecnologia e Química Fina – Laboratório Associado, Escola Superior de Biotecnologia, Rua Arquiteto Lobão Vital, Porto, Portugal, QOPNA, Departamento de Química, Universidade de Aveiro, Aveiro, Portugal, Instituto de Biologia Experimental e Tecnológica, Avenida da República, Quinta-do-Marquês, Estação Agronómica Nacional, Oeiras, Portugal
Methods: GC-MS, biological assays, HPLC, methylation analysis, TFA hydrolysis, ANOVA
The publication contains the following compound(s):
- Compound ID: 347
Structure type: oligomer
Trivial name: 1-kestose, inulin
Compound class: fructan, fructo-oligosaccharide
Reference(s) to other database(s): GTC:G11260CN, GlycomeDB:
396, CCSD:
26122, CBank-STR:5624, GenDB:MH445969, US-PT:US2017298332A1
- Compound ID: 21786
Structure type: fragment of a bigger structure
Trivial name: inulobiose
Reference(s) to other database(s): GTC:G99327ZM
- Compound ID: 21787
Structure type: fragment of a bigger structure
Trivial name: blastose
Reference(s) to other database(s): GTC:G08398AS
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